conjugate
unconjugated
antibody form
purified antibody
clone
2E9, monoclonal
purified by
using protein G
concentration
(Please refer to lot specific datasheet.)
technique(s)
ELISA: suitable, immunohistochemistry: suitable, western blot: suitable
isotype
IgG1κ
UniProt accession no.
target post-translational modification
unmodified
species reactivity
mouse
General description
Full length recombinant human 2-5A-dependent ribonuclease (RNase L).
~83.53 kDa calculated.
Application
Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution from a representative lot detected RNase L in human stomach tissue sections.
Western Blotting Analysis: A representative lot detected RNase L in Western Blotting applications (Dong, B., et. al. (1995). J Biol Chem. 270(8):4133-7).
ELISA Analysis: A representative lot detected RNase L in ELISA applications (Dong, B., et. al. (1995). J Biol Chem. 270(8):4133-7).
Immunohistochemistry Analysis: A representative lot detected RNase L in Immunohistochemistry applications (Wang, L., et. al. (1995). Clin Cancer Res. 1(11):1421-8).
Western Blotting Analysis: A representative lot detected RNase L in Western Blotting applications (Dong, B., et. al. (1995). J Biol Chem. 270(8):4133-7).
ELISA Analysis: A representative lot detected RNase L in ELISA applications (Dong, B., et. al. (1995). J Biol Chem. 270(8):4133-7).
Immunohistochemistry Analysis: A representative lot detected RNase L in Immunohistochemistry applications (Wang, L., et. al. (1995). Clin Cancer Res. 1(11):1421-8).
Biochem/physiol Actions
Clone 2E9 is a mouse monoclonal antibody that detects Ribonuclease L in human cells.
Physical form
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Immunohistochemistry (Paraffin) in human prostate tissue sections.
Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution of this antibody detected RNase L in human prostate tissue sections.
Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution of this antibody detected RNase L in human prostate tissue sections.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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