Anti-RhoB, clone 9G5

Anti-RhoB, clone 9G5, Cat. No. MABS2178, is a mouse monoclonal antibody that detects Rho-related GTP-binding protein RhoB and has been tested for use in Flow Cytometry.

Flow Cytometry

Clone 9G5 is a mouse monoclonal antibody that detects RhoB. it targets an epitope within 19 amino acids from the C-terminal half.

KLH-conjugated linear peptide corresponding to 19 amino acids from the C-terminal half of human Rho B.

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Purified mouse monoclonal antibody IgG2a in PBS without azide.

Evaluated by Flow Cytometry in HeLa cells.

Flow Cytometry Analysis: 1 µg of this antibody detected RhoB in one million HeLa cells.

Rho-related GTP-binding protein RhoB (UniProt: P62745; also known as Rho cDNA clone 6, h6) is encoded by the RHOB (also known as ARH6, ARHB) gene (Gene ID: 388) in human. RhoB is a member of the Ras super-family of small GTP-binding proteins, which is involved in hydrolysis of GTP and is active in GTP-bound form. RhoB is a short-lived Rho GTPase whose expression is inducible by a variety of stimuli including growth factors and stress stimuli. Its levels are up-regulated by DNA damaging agents, such as hydrogen peroxide or ionizing radiation. RhoB plays a negative role in tumorigenesis as its deletion is known to cause tumor formation. It is reported to mediate apoptosis in neoplastically transformed cells following DNA damage. It is not considered to be essential for development, but affects cell adhesion and growth factor signaling in transformed cells. RhoB displays 83% homology with RhoA. However, unlike RhoA, which is cytosolic and translocates to plasma membrane upon activation, RhoB localizes to endosomes/multivesicular bodies. It participates in sorting and degradation of growth factors and cytokine receptors. RhoB has three nucleotide-binding regions (aa 12-19; 59-63; and 117-120). RhoB is shown to be critically required for the inflammatory response of endothelial cells to TNF alpha, possibly through MAP kinase activation downstream of the TNFR.

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