Anti-ZnT8/SLC30A8 Antibody, clone mAb20

Zinc transporter 8 (UniProt: Q8IWU4; also known as Proton-coupled zinc antiporter SLC30A8, ZnT-8, Solute carrier family 30 member 8) is encoded by the SLC30A8 (also known as ZNT8) gene (Gene ID: 169026) in human. Zinc is required for insulin crystallization, storage, and secretion in the secretory granules of pancreatic β-cells. Delivery of Zn2+ into the insulin-containing granules is largely accomplished by the ZnT8 protein. ZnT8 is a homodimeric, multi-pass membrane protein with four cytoplasmic domains, six transmembrane domains, and three extracellular domains. It serves as a granule-specific Zn2+ transporter that is abundantly expressed in pancreatic islet cells. ZnT-8 expression is mostly found in insulin-producing beta cells and is also expressed in peripheral blood mononuclear cells, including T-cells and B-cells. It contains a histidine-rich region, HXXXXXHNH-motif, in its third cytoplasmic domain, which serves as a ligand for zinc. It plays a major role in Zn2+ uptake into the secretory granules. During stimulated secretion, Zn2+ is shown to be co-released with other granular content into the extracellular medium.Loss of ZnT8 function is reported to reduce total islet Zn2+ content as well as free Zn2+ in the cytosol and granules. Loss of ZnT8 also results in reduced dense core granules and increased granule diameter. Studies have shown that the expression of ZnT8 is significantly reduced in the islets of diabetic murine models. Two isoforms of ZnT8 have been described that are produced by alternative splicing. This mAb20 mouse monoclonal specifically detects ZnT8/SLC30A8 and is suitable for multiple immunodetection applications. (Ref.: Merriman, C., et al. (2018). J Biol Chem. 293(42):16206-16216; Ghazvini Zadeh, EH., et al. (2020). Cell Reports 32(2); 107904; Dwivedi, OP., et al. (2019). Nat. Genetics. 51(11); 1596-1606).

Human ZnT8 antigen (the Arg-325 variant) in reconstituted proteoliposomes.

Quality Control Testing

Evaluated by Immunohistochemistry in human pancreas tissue sections

Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution from a representative lot detected ZnT8/SLC30A8 in human pancreas tissue sections.

Tested Applications

Western Blotting Analysis: A representative lot detected ZnT-8 /SLC30A8 in Western Blotting application (Merriman, C., et al. (2018). J Biol Chem. 293(42):16206-16216).

Immunocytochemistry Analysis: A representative lot detected ZnT-8 /SLC30A8 in Immunocytochemistry application (Merriman, C., et al. (2018). J Biol Chem. 293(42):16206-16216).

ELISA Analysis: A representative lot detected ZnT-8 /SLC30A8 in ELISA application (Merriman, C., et al. (2018). J Biol Chem. 293(42):16206-16216).

Flow Cytometry Analysis: A representative lot detected ZnT-8 /SLC30A8 in Flow Cytometry application (Merriman, C., et al. (2018). J Biol Chem. 293(42):16206-16216).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Clone mAb20 is a mouse monoclonal antibody that specifically detects ZnT8/SLC30A8.

Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Recommended storage: +2°C to +8°C.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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