Anti-Jagged2 Antibody, clone HMJ2-1

Protein jagged-2 (UniProt: Q9QYE5; also known as Jagged2) is encoded by the Jag2 gene (Gene ID:16450) in mouse. The Notch signaling pathway is an intercellular signaling mechanism that is essential for proper embryonic development. Members of the Notch protein family are transmembrane receptors that are critical for various cell fate decisions. Jagged2 is one of several ligands that activate Notch and related receptors. It is a single-pass type I membrane protein and is synthesized with a signal peptide sequence (aa 1-23), the removal of which yields the mature Jagged2 with a large extracellular domain (aa 24-1082), a transmembrane domain (aa 1083-1103) and, a cytoplasmic domain (aa 1104-1247). Jagged2 expression varies across different tissues and developmental stages and is found to be highest in fetal thymus, epidermis, foregut dorsal root ganglia and inner ear. In 2-weeK-old mice its abundantly found in heart, lung, thymus, skeletal muscle, brain and testis. Its expression overlaps partially with Notch1 expression. Jagged2 has been implicated in various diseases, mainly due to its role in the Notch signaling pathway. The interaction of Natural Killer (NK) cells with dendritic cells (DC) contributes to NK cell-mediated antitumor or antimicrobial responses. Studies show an involvement of Jagged2 Notch interaction in augmenting NK cell cytotoxicity mediated by dendritic cells and indicated that, the Jagged2 Notch axis plays a crucial role in DC-mediated NK cell cytotoxicity. It is also suggested that manipulation of this interaction may provide an approach to induce potent tumor immunity or to inhibit certain autoimmune diseases caused by NK cell activation. This clone functions as an agonist by promoting Treg cell differentiation. Aberrant Jagged2 expression can lead to uncontrolled cell proliferation and tumorigenesis in cancers such as breast cancer, lung cancer, and glioblastoma. (Ref.: Kijima, M., et al. (2008). Proc Natl Acad Sci USA. 105(19):7010-5; Elyaman, W., et al. (2012). Immunity. 36(4):623-34; Riella, L.V., et al. (2013). Eur J Immunol. 43(6):1449-58; Sekine, C., et al. (2014). Arthritis Rheumatol. 66(10):2751-61).

CHO cells overexpressing mouse Jagged2

Quality Control Testing

Evaluated by Flow Cytometry in CHO cells transfected with mouse Jagged2.

Flow Cytometry Analysis: 1.0 g of this antibody detected Jagged2 in one million CHO cells transfected with mouse Jagged2.

Tested Applications

Flow Cytometry Analysis: A 1:500 dilution from a representative lot detected Jagged2 in CHO cells transfected with mouse Jagged2 (Courtesy of Dr. Hideo Yagita, Ph.D., Juntendo University, Japan).

ELISA: A representative lot detected Jagged2 in ELISA application (Sekine, C., et al. (2014). Arthritis Rheumatol. 66(10):2751-61).

Flow Cytometry Analysis: A representative lot detected Jagged2 in Flow Cytometry application (Kijima, M., et al. (2008). Proc Natl Acad Sci USA. 105(19):7010-5).

Agonist: A representative lot promoted Treg cell differentiation and enhanced Jagged-2 Notch signaling via activation of RBP-Jk (canonical Notch pathway). (Elyaman, W., et al. (2012). Immunity. 36(4):623-34; Riella, L.V., et al. (2013). Eur J Immunol. 43(6):1449-58).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Clone HMJ2-1 is an Armenian Hamster monoclonal antibody that specifically detects Jagged2.

Purified Armenian hamster monoclonal antibody in PBS without azide.

0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.