Anti-ALDH3A1 Antibody, clone 247 H9D4F5

Aldehyde dehydrogenase, dimeric NADP-preferring (UniProt: P30838; EC:1.2.1.5; also known as ALDHIII, Aldehyde dehydrogenase 3, Aldehyde dehydrogenase family 3 member A1) is encoded by the ALDH3A1 (also known as ALDH3) gene (Gene ID: 218) in human. ALDH3A1 is a member of the aldehyde dehydrogenase superfamily that catalyzes the NAD(P)+ -dependent oxidation of aromatic and medium chain aldehydes. ALDH3A1 protein forms a homodimer. ALDH3A1 is one of the most abundant proteins expressed in cornea, but it is expressed in other tissues as well, including the stomach, esophagus, skin and lung. ALDH3A1 expression is inversely regulated by PPARg (Peroxisome Proliferators-Activated Receptor g) and is directly correlated with cell proliferation. It plays a role in protection of corneal cells against cellular oxidative stress, including UV radiation and functions as an antioxidant. (Ref.: Pappa, A., et al. (2003). Biochem J. 376(Pt 3):615-23.; Pappa, A., et al. (2005). J Biol Chem. 280(30):27998-8006.; Lassen, N., et al. (2006). Free Radic Biol Med. 41(9):1459-69.; Oraldi, M., et al. (2011). Chem Biol Interact. 191(1-3):171-6.; Black, W., et al. (2012). Free Radic Biol Med. 52(9):1937-44.)

Purified, full length, recombinant human ALDH3A1 protein.

Quality Control Testing

Evaluated by Western Blotting in A549 cell lysate.

Western Blotting Analysis: A 1:500 Dilution of this antibody detected ALDH3A1 in A549 cell lysates.

Tested Applications

Western Blotting Analysis: A representative lot detected ALDH3A1 in Western Blotting applications (Pappa, A., et al. (2003). Biochem J. 376(Pt 3):615-23; Pappa, A., et al. (2005). J Biol Chem. 280(30):27998-8006; Lassen, N., et al. (2006). Free Radic Biol Med. 41(9):1459-69; Oraldi, M., et al. (2011). Chem Biol Interact. 191(1-3):171-6; Black, W., et al. (2012). Free Radic Biol Med. 52(9):1937-44).

Immunofluorescence Analysis: A representative lot detected ALDH3A1 in Immunofluorescence applications (Pappa, A., et al. (2003). Biochem J. 376(Pt 3):615-623; Pappa, A., et al. (2005). J Biol Chem. 280(30):27998-28006).

Immunohistochemistry Analysis: A representative lot detected ALDH3A1 in Immunohistochemistry applications (Pappa, A., et al. (2003). Biochem J. 376(Pt 3):615-623; Pappa, A., et al. (2005). J Biol Chem. 280(30):27998-28006).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Clone H9D4F5 is a mouse monoclonal antibody that detects ALDH3A1. It targets an epitope within the human ALDH3A1 protein.

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Recommended storage: +2°C to +8°C.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.