Anti-SCUBE1 Antibody, clone 701

Signal peptide, CUB and EGF-like domain-containing protein 1 (UniProt: Q8IWY4) is encoded by the SCUBE1 gene (Gene ID: 80274) in human. The gene encodes a platelet-endothelial cell surface protein that is expressed during early embryogenesis. The protein plays a role during the early stages of central nervous system development. SCUBE1 is a novel adhesive molecule that mediates platelet matrix interaction and ristocetin-induced platelet agglutination. It is produced with a signal peptide sequence at the N-terminus (aa 1-23) followed by nine tandemly arranged epidermal growth factor (EGF)-like repeats (aa 33-402), a spacer region and three cysteine-rich repeat motifs and a CUB domain at the C-terminus (aa 798-910). SCUBE1 protein is highly expressed in platelets and stored in the platelet -granules, and translocated to the surface upon platelet stimulation. Studies suggest that matrix-bound and soluble fragments of SCUBE1 may play critical (patho)physiological roles in cardiovascular biology. Platelet activation plays a crucial role in acute coronary syndrome (ACS) and acute ischemic stroke (AIS), and platelet stimulation is associated with increased plasma concentration of SCUBE1. Studies show plasma SCUBE1 concentration is significantly elevated in ACS and AIS but, not in coronary artery disease (CAD) patients. Plasma SCUBE1 is implicated to be a potential biomarker of platelet activation in acute thrombotic disease. It may also function in renal regeneration and repair. (Ref.: Tu, C-F., et al. (2006). Cardiovasc Res. 71(3):486-95; Dai, D-F, et al. (2008). J Am Coll Cardiol. 51(22):2173-80; Liao, W-J, et al. (2019). Biochim Biophys Acta Mol Basis Dis. 1865(2):329-338).

GST-tagged, recombinant fragment from the C-terminus of human SCUBE1.

Quality Control Testing

Evaluated by Western Blotting in HEK-293T cells transfected with pFlag-hSCUBE1.

Western Blotting Analysis: A 1:500 dilution from a representative lot detected SCUBE1 in lysate from HEK-293T cells transfected with pFlag-hSCUBE1.

Tested Applications

Western Blotting Analysis: A representative lot detected SCUBE1 in Western Blotting application (Tu, C-F., et al. (2006). Cardiovasc Res. 71(3):486-95; Dai, D-F, et al. (2008). J Am Coll Cardiol. 51(22):2173-80; Liao, W-J, et al. (2019). Biochim Biophys Acta Mol Basis Dis. 1865(2):329-338).

Immunofluorescence Analysis: A representative lot detected SCUBE1 in Immunofluorescence application (Tu, C-F., et al. (2006). Cardiovasc Res. 71(3):486-95).

Flow Cytometry Analysis: A representative lot detected SCUBE1 in Flow Cytometry application (Tu, C-F., et al. (2006). Cardiovasc Res. 71(3):486-95).

Immunohistochemistry Analysis: A representative lot detected SCUBE1 in Immunohistochemistry application (Tu, C-F., et al. (2006). Cardiovasc Res. 71(3):486-95; Liao, W-J, et al. (2019). Biochim Biophys Acta Mol Basis Dis. 1865(2):329-338).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Clone 701 is a mouse monoclonal antibody that specifically detects SCUBE1 but not, SCUBE2 or SCUBE3. It targets an epitope within 200 amino acids from the C-terminal region.

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Recommended storage: +2°C to +8°C.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.