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Merck
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About This Item

UNSPSC代码:
12352203
eCl@ss:
32160702
NACRES:
NA.41
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生物来源

mouse

质量水平

偶联物

unconjugated

抗体形式

purified antibody

抗体产品类型

primary antibodies

克隆

4H3, monoclonal

种属反应性

human

技术

ELISA: suitable
western blot: suitable

同位素/亚型

IgG1κ

NCBI登记号

UniProt登记号

运输

wet ice

靶向翻译后修饰

unmodified

基因信息

human ... C3(718)

一般描述

The protein called Acylation stimulating protein (ASP) is a cleavage fragment protein from the Complement C3 gene. C3 plays a central role in the activation of the complement system. C3’s processing by C3 convertase is the central reaction in both classical and alternative complement pathways. ASP acts as an adipogenic hormone that stimulates triglyceride synthesis and glucose transport in adipocytes, and thus it helps regulate fat storage and triglyceride synthesis. ASP stimulates triglyceride synthesis via PLC, MAPK and AKT signaling pathways and ASP promotes the phosphorylation and internalization and recycling of Complement factor C5AR2. ASP is expressed in adipocytes and released into the plasma during both the fasting and postprandial periods. Increased levels of C3 and its cleavage product ASP are associated with obesity, diabetes and coronary heart disease. Short-term endurance training reduces baseline ASP levels and subsequently fat storage.
~ 16/10 kDa observed, 10 kDa indicates native ASP, 16 kDa indicates HIS-Tag ASP. HIS-Tag was added for purification purposes. We have found no difference in Activity (measured by TGS, Fatty Acid uptake, etc) between rASP with/without the tag. Further, the ELISA was successful in measuring both.

免疫原

linear peptide corresponding to Human Acylation Stimulating Protein.

应用

ELISA Analysis: 7 µg/mL of this antibody detected ASP (PEG precipitated) in a sandwich ELISA format (Saleh, J,. et al, J. Lipid Res. 1998. 39: 884–891)
Research Category
Signaling

Metabolism
Research Sub Category
Insulin/Energy Signaling

Lipid Metabolism & Weight Regulation
This Anti-Acylation Stimulating Protein Antibody, clone 4H3 is validated for use in Western Blotting and ELISA for the detection of Acylation Stimulating Protein.

外形

Protein G Purified
Format: Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

制备说明

Stable for 1 year at 2-8°C from date of receipt.

分析说明

Evaluated by Western Blotting on purified Acylation Stimulating Protein from transfected HEK293 cell lysate

Western Blotting Analysis: 0.1 µg/mL of this antibody detected purified Acylation Stimulating Protein from transfected HEK293 cell.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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储存分类代码

12 - Non Combustible Liquids

WGK

WGK 1


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