Purified recombinant fragment of human PRKAA1 expressed in E. Coli.

~65 kDa observed. Uncharacterized bands may be observed in some lysate(s).

Immunohistochemistry Analysis (IHC): A 1:200-1,000 dilution from a representative lot detected PRKAA1 in ovarian cancer and brain tissues.

Immunocytochemistry Analysis (ICC): A 1:200-1,000 dilution from a representative lot detected PRKAA1 in Ntera-2 cells.

Flow Cytometry Analysis (FC): A 1:200-400 dilution from a representative lot detected PRKAA1 in PC-2 cells.

ELISA: A representative lot detected control antigen (100 ng) and antigen (10 ng, 50 ng, 100 ng) in ELISA (Direct).

Mouse monoclonal IgG1ascitic fluid containing 0.03% sodium azide.

Stable for 1 year at -20°C from date of receipt.Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Evaluated by Western Blotting in Jurkat, HeLa, HepG2, MCF-7, Cos7, NIH/3T3, K562, HEK293, and PC-12 cell lysates.

Western Blotting Analysis (WB): A 1:500-2,000 dilution of this antibody detected PRKAA1 in Jurkat, HeLa, HepG2, MCF-7, Cos7, NIH/3T3, K562, HEK293, and PC-12 cell lysates.

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