Anti-Tropomyosin 3 Antibody, clone 2G10.2

Tropomyosin alpha-3 chain (UniProt: Q63610; also known as Gamma-tropomyosin, Tropomyosin-3, Tropomyosin-5) is encoded by the Tpm3 (also known as Tpm-5, Tpm5) gene (Gene ID: 117557) in rat. The TPM3 gene codes for the slow-twitch skeletal muscle isoform ( s Tm) and at least 9 LMW cytoskeletal isoforms referred to as Tm5NM1 to Tm5NM11. Tropomyosins are dimers of coiled-coil proteins that provide stability to actin filaments and regulate access of other actin-binding proteins. In muscle cells, they regulate muscle contraction by controlling the binding of myosin heads to the actin filament. In non-muscle cells tropomyosins are implicated in stabilizing cytoskeleton actin filaments. Tropomyosins have been implicated in the pathogenesis of cancer where high molecular weight isoforms are consistently down-regulated in transformed cells, while malignant cells display an increased reliance on low molecular weight isoforms. Tropomyosin 3 is a homodimeric protein that can form heterodimers with a beta (TPM2) chain. Its coiled coil structure is formed by 2 polypeptide chains. (Ref.: Glass, JJ et al. (2015). BMC Cancer. 15; 712).

Diphtheria Toxoid Carrier Protein-conjugated linear peptide corresponding to 27 amino acids from the C-terminal region of rat Tpm3.1 and Tpm3.2

Western Blotting Analysis: 1 µg/mL from a representative lot detected Tropomyosin 3 in human lung tissue lysate.

Immunofluorescence Analysis: A representative lot detected Tropomyosin 3 in Immunofluorescence applications (Schevzov, G., et. al. (2011). Bioarchitecture. 1(4):135-164).

Western Blotting Analysis: A representative lot detected Tropomyosin 3 in Western Blotting applications (Schevzov, G., et. al. (2011). Bioarchitecture. 1(4):135-164).

Clone 2G10.2 is a mouse monoclonal antibody that specifically detects Tpm3.1 and Tpm3.2. It targets an epitope within 27 amino acids from the C-terminal region.

Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Evaluated by Western Blotting in mouse liver tissue lysate. Western Blotting Analysis: 1 µg/mL of this antibody detected Tropomyosin 3 in mouse liver tissue lysate.

Concentration: Please refer to lot specific datasheet.

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