MABT1353
Anti-C-Nap1 (Cep250) Antibody, clone 150-230-1
clone 150-230-1, from mouse
别名:
Centrosome-associated protein CEP250, 250 kDa centrosomal protein, Cep250, Centrosomal Nek2-associated protein 1, C-Nap1, Centrosomal protein 2
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关于此项目
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
150-230-1, monoclonal
Application:
IF, WB
Citations:
1
biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
150-230-1, monoclonal
species reactivity
human
packaging
antibody small pack of 25 μg
technique(s)
immunofluorescence: suitable, western blot: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
target post-translational modification
unmodified
Gene Information
human ... CEP250(11190)
General description
Centrosome-associated protein CEP250 (UniProt: Q9BV73; also known as 250 kDa centrosomal protein, Cep250, Centrosomal NEK2-associated protein 1, C-Nap1, Centrosomal protein 2) is encoded by the CEP250 (also known as CEP2, CNAP1) gene (Gene ID: 11190) inn human. Cep250 is a component of the core centrosome that is ubiquitously expressed. It can exist as a monomer and homodimer. It specifically associates with the proximal ends of both mother and daughter centrioles during interphase and in mitotic cells it dissociates from the mitotic spindle poles. At the end of cell division, it is shown to re-accumulate at centrosomes. Cep250 is differentially phosphorylated during cell cycle and phosphorylation status regulates its association/dissociation from centrosome. During M phase its C-terminal part is shown to be phosphorylated by NEK2. Two isoforms of Cep250 have been described that are produced by alternative splicing. Loss of Cep250-mediated centriole cohesion leads to an altered cell migration phenotype.
~280 kDa observed; 281.14 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Immunogen
Recombinant fragment corresponding to 455 amino acids from the C-terminal region of human Centrosome-associated protein CEP250.
Application
Anti-C-Nap1 (Cep250), clone 150-230-1, Cat. No. MABT1353, is a highly specific mouse monoclonal antibody that targets Centrosome-associated protein CEP250 and has been tested for use in Immunofluorescence and Western Blotting.
Immunofluorescence Analysis: A representative lot detected C-Nap1 (Cep250) in Immunofluorescence applications (Fava, L.L., et. al. (2017). Genes Dev. 31(1):34-45).
Western Blotting Analysis: A representative lot detected C-Nap1 (Cep250) in HeLa S3 cell lysates. (Courtesy of Prof. Erich A. Nigg, Ph.D., Biozentrum, University of Basel, Switzerland).
Western Blotting Analysis: A representative lot detected C-Nap1 (Cep250) in HeLa S3 cell lysates. (Courtesy of Prof. Erich A. Nigg, Ph.D., Biozentrum, University of Basel, Switzerland).
Biochem/physiol Actions
Clone 150-230-1 is a mouse monoclonal antibody that detects human Centrosome-associated protein CEP250 (Cep20). It targets an epitope within 455 amino acids from the C-terminal region.
Physical form
Format: Purified
Analysis Note
Isotype testing: Identity Confirmation by Isotyping Test.
Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be mouse IgG1.
Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be mouse IgG1.
Other Notes
Concentration: Please refer to lot specific datasheet.
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存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Selahattin Can Ozcan et al.
Scientific reports, 14(1), 4370-4370 (2024-02-23)
Centrosome amplification is a hallmark of cancer and PLK4 is one of the responsible factors for cancer associated centrosome amplification. Increased PLK4 levels was also shown to contribute to generation of cells with centriole amplification in mammalian tissues as olfactory
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