Anti-Claudin-4 Antibody, clone 10B4.3

Claudin 4, also known as Clostridium perfringens enterotoxin receptor, or CPE-R, or CPE-receptor or Williams-Beuren syndrome chromosomal region 8 protein, and encoded by the gene CLDN4/CPER/CPETR1/WBSCR8, plays a critical role in tight junction specific obliteration of intercellular space. Claudin 4 directly interacts with several important tight junction associated proteins including ZO-1,2,3 and EPHA2 proteins, and through these interactions, Claudin 4 regulates tight junction formation. Claudin 4 is localized to cell junctions and membranes at tight junctions. Claudin 4 is expressed in most tissues particularly in intestine, liver, and pancreas. Defects in Claudin 4 may be associated with Williams Beuren syndrome genetic deletion syndrome. Claudin 4 is also over expressed in many cancers and thus is a target of anti-cancer research using C. perfringens enterotoxin.

~ 22 kDa observed

Epitope: Cytoplasmic domain

KLH-conjugated linear peptide corresponding to the cytoplasmic domain of human Claudin-4.

Anti-Claudin-4 Antibody, clone 10B4.3 is an antibody against Claudin-4 for use in western blotting & IHC (Paraffin).

Research Category
Cell Structure

Research Sub Category
ECM Proteins

Western Blotting Analysis: 2.0 µg/mL from a representative lot detected Claudin-4 in 10 µg of MCF-7 cell lysate.

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Claudin-4 in human ileum and human tonsil tissue.

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected Claudin-4 in 10 µg of HeLa cell lysate.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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