biological source
mouse
conjugate
unconjugated
Quality Level
Gene Information
human ... PARVA(55742)
antibody product type
primary antibodies
clone
3B5, monoclonal
mol wt
calculated mol wt 42.24 kDa, observed mol wt ~51 kDa
purified by
using protein G
species reactivity
human, mouse
packaging
antibody small pack of 100 μg
technique(s)
ELISA: suitable, western blot: suitable
isotype
IgG1κ
epitope sequence
N-terminal half
Protein ID accession no.
UniProt accession no.
shipped in
ambient
target post-translational modification
unmodified
General description
-Parvin (UniProt: Q9NVD7; also known as Actopaxin, CH-ILKBP, Calponin-like integrin-linked kinase-binding protein, Matrix-remodeling-associated protein 2) is encoded by the PARVA (also known as MXRA2) gene (Gene ID: 55742) in human. -Parvin is a widely e
Immunogen
GST-tagged recombinant fragment corresponding to 344 amino acids from human -Parvin (PARVA).
Application
Quality Control Testing
Evaluated by Western Blotting in lysate from HEK293T cells overexpressing -Parvin (PARVA).
Western Blotting Analysis (WB): A 1:500 dilution of this antibody detected -Parvin in lysate from HEK293T cells overexpressing -Parvin (PARVA).
Tested Applications
Western Blotting Analysis: A representative lot detected -Parvin/PARVA in Western Blotting applications (Tu, Y., et al. (2001). J Cell Biol.;153(3):585-98; Zhang, Y., et al. (2004). J Biol. Chem. 279(40):41695-705).
ELISA Analysis: A representative lot detected -Parvin/PARVA in ELISA applications (Tu, Y., et al. (2001). J Cell Biol. 153(3):585-98).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Western Blotting in lysate from HEK293T cells overexpressing -Parvin (PARVA).
Western Blotting Analysis (WB): A 1:500 dilution of this antibody detected -Parvin in lysate from HEK293T cells overexpressing -Parvin (PARVA).
Tested Applications
Western Blotting Analysis: A representative lot detected -Parvin/PARVA in Western Blotting applications (Tu, Y., et al. (2001). J Cell Biol.;153(3):585-98; Zhang, Y., et al. (2004). J Biol. Chem. 279(40):41695-705).
ELISA Analysis: A representative lot detected -Parvin/PARVA in ELISA applications (Tu, Y., et al. (2001). J Cell Biol. 153(3):585-98).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti- -Parvin/PARVA, clone 3B5, Cat. No. MABT1440, is a mouse monoclonal antibody that detects -Parvin and is tested for use in ELISA and Western Blotting.
Biochem/physiol Actions
Clone 3B5 is a mouse monoclonal antibody that detects a-Parvin (PARVA). It targets an epitope within the N-terminal half.
Physical form
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Recommended storage: +2°C to +8°C.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
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