Anti-COL4A6 Antibody, clone 122-2

Collagen type IV alpha 6 chain (UniProt: A0A0G2K601; also known as Col4a6) is encoded by the Col4a6 gene (Gene ID: 363458) in rat. The collagen super family includes over 20 different types of collagens with at least 38 distinct polypeptide chains. Of these types I, II, and III are the most abundant and are involved in the formation of fibrils. Type IV collagen forms a two-dimensional reticulum and is a major component of the basal lamina. It is a network-forming collagen that underlies epithelial and endothelial cells and functions as a barrier between tissue compartments. Type IV collagen has many binding partners and forms the backbone of the basement membrane. It also contains numerous cysteine residues that are involved in inter- and intramolecular disulfide bonding. The type IV collagen family comprises six isomeric chains, designated 1(IV) to 6(IV), each of which can form a triple helix structure with 2 other chains to generate type IV collagen network. Each type of collagen IV is a heterotrimer composed of three chains. Formation of these heterotrimers is initiated by the non-collagenous 1 (NC1) domain interactions and is accompanied by folding of the collagenous domains into triple helices. Collagen IV 6 is synthesized with a signal peptide (aa 1-20), which is subsequently cleaved off to generate the mature form. Clone 122-2 specifically recognizes collagen IV 6 and reacts with the NC1 domain. (Ref.: Namba, M., et al. (2021). Sci. Rep. 11; 20836; Khoshnoodi, J., et al. (2008). Microsc. Res. Tech. 71(5); 357-370).

His-tagged recombinant fragment corresponding to 231 amino acids from the C-terminal region of rat Collagen type IV 6 chain (COL4A6). It was injected into Col4a5 deficient male rats.

Quality Control Testing

Evaluated by Western Blotting in GST-tagged recombinant COL4A6.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected GST-tagged recombinant NC1 domain of Rat COL4A6.

Tested Applications

Immunohistochemistry Applications: A representative lot detected COL4A6 in Immunohistochemistry applications (Namba, M., et al. (2021). Sci Rep. 11(1):20836).

Western Blotting Analysis: A representative lot detected Col4a6 in Western Blotting applications (Namba, M., et al. (2021). Sci Rep. 11(1):20836).

ELISA Analysis: A representative lot detected Col4a6 in ELISA applications (Namba, M., et al. (2021). Sci Rep.11(1):20836).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Clone 122-2 is a rat monoclonal antibody that detects Collagen type IV 6 chain (COL4A6). It targets an epitope within the C-terminal region.

Purified rat monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Recommended storage: +2°C to +8°C.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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