conjugate
unconjugated
antibody form
purified antibody
clone
2B12, monoclonal
purified by
using protein G
concentration
(Please refer to lot specific datasheet.)
species reactivity
mouse
technique(s)
flow cytometry: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable
isotype
IgG2aκ
UniProt accession no.
mode of action
| inhibits
target post-translational modification
unmodified
General description
23.15 kDa calculated.
An artificial membrane protein consisting of the extracellular region of human Claudin 5.
Application
Epitope: extracellular domain
Biochem/physiol Actions
Clone 2B12 is a mouse monoclonal antibody that detects human Claudin 5. It targets an epitope within nine amino acids in the second extracellular domain.
Physical form
Purified mouse monoclonal antibody IgG2a in PBS without azide.
Preparation Note
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Evaluated by Flow Cytometry in human umbilical vein endothelial cells (HUVEC).
Flow Cytometry Analysis: 1 µg of this antibody detected Claudin 5 one million human umbilical vein endothelial cells (HUVEC).
Flow Cytometry Analysis: 1 µg of this antibody detected Claudin 5 one million human umbilical vein endothelial cells (HUVEC).
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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