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Merck
CN

MABT29

Anti-Ninein Antibody, clone 79-160-7

clone 79-160-7, from mouse

别名:

ninein (GSK3B interacting protein), Glycogen synthase kinase 3 beta-interacting protein, ninein centrosomal protein, GSK3B-interacting protein

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关于此项目

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
79-160-7, monoclonal
Application:
IP, WB
Citations:
10
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biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

purified antibody

antibody product type

primary antibodies

clone

79-160-7, monoclonal

species reactivity

human

technique(s)

immunoprecipitation (IP): suitable, western blot: suitable

isotype

IgG2aκ

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... NIN(51199)

General description

Ninein is a coiled-coil, centrosomal protein that is a necessary component in microtubule minus-end anchorage and positioning, and may have a role as a maturation factor for centrosomes. It may also be involved in microtubule nucleation. More recent studies have identified ninein as a crucial component in the formation of blood vessels. Expression of ninein is ubiquitous, but significantly higher levels have been observed in skeletal muscle and heart tissue. There have been 8 isoforms characterized due to alternative splicing.
~ 250 kDa observed MW. An unidentified nonspecific band appears at ~ 50 kDa

Immunogen

Recombinant protein corresponding to human Ninein.

Application

Anti-Ninein Antibody, clone 79-160-7 is an antibody against Ninein for use in WB & IP.
Immunocytochemistry Analysis: 1:500 dilution of the antibody has been shown to detect Ninein in HeLa and A431 cells.

Immunoprecipitation Analysis: The antibody has been shown to immunoprecipitate Ninein in L929 cells. (Delgehyr, 2005).

Physical form

Format: Purified
Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine, pH 7.4, 150 mM NaCl with 0.05% sodium azide.

Analysis Note

Evaluated by Western Blot in Hek293 cell lysate.

Western Blot Analysis: 1 µg/mL of the antibody detected Ninein in 10 µg of Hek293 cell lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.


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存储类别

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable



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Izumi Dateyama et al.
Journal of cell science, 132(2) (2018-12-24)
The primary cilium, a solitary protrusion from most mammalian cells, functions as a cell sensor by receiving extracellular signals through receptors and channels accumulated in the organelle. Certain G-protein-coupled receptors (GPCRs) specifically localize to the membrane compartment of primary cilia.
Aamir Ali et al.
Nature communications, 14(1), 289-289 (2023-01-27)
Organization of microtubule arrays requires spatio-temporal regulation of the microtubule nucleator γ-tubulin ring complex (γTuRC) at microtubule organizing centers (MTOCs). MTOC-localized adapter proteins are thought to recruit and activate γTuRC, but the molecular underpinnings remain obscure. Here we show that
Tatsuo Miyamoto et al.
The EMBO journal, 39(12), e103499-e103499 (2020-05-06)
Primary cilia are antenna-like organelles on the surface of most mammalian cells that receive sonic hedgehog (Shh) signaling in embryogenesis and carcinogenesis. Cellular cholesterol functions as a direct activator of a seven-transmembrane oncoprotein called Smoothened (Smo) and thereby induces Smo



全球贸易项目编号

货号GTIN
MABT2904053252479465