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Merck
CN

MABT332

Anti-Obscurin Antibody, clone 4A8

clone 4A8, from mouse

别名:

Obscurin, Obscurin-RhoGEF, Obscurin-myosin light chain kinase, Obscurin-MLCK

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关于此项目

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
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biological source

mouse

antibody form

purified antibody

antibody product type

primary antibodies

clone

4A8, monoclonal

species reactivity

rat

species reactivity (predicted by homology)

human (based on 100% sequence homology)

technique(s)

immunohistochemistry: suitable (paraffin), western blot: suitable

isotype

IgG1κ

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... OBSCN(84033)

General description

800 kDa calculated
Obscurin, also known as Obscurin-RhoGEF or Obscurin-myosin light chain kinase, is encoded by the gene name OBSCN. Obscurin is involved in myofibrillogenesis and the assembly of myosin into sarcomeric A bands in striated muscle. Obscurin isoform 3 plus ANK1 isoform Mu17/Ank1.5 may be molecular link between the sarcoplasmic reticulum and myofibrils.

Immunogen

GST-tagged recombinant protein corresponding to the N-terminus of human Obscurin.

Application

This Anti-Obscurin antibody is validated for use in WB, IH(P) for the detection of Obscurin.
Western Blotting Analysis: A representative lot detected Obscurin in extracts of adult rat quadriceps tissue lysate (Bowman, A.L., et al. (2007). FEBS Letters. 581:1549-1554).
Immunohistochemistry by fluorescent Analysis: A representative lot detected Isocitrate dehydrogenase in rat muscle tissue at resting stage (Bowman, A.L., et al. (2007). FEBS Letters. 581:1549-1554).

Physical form

Format: Purified

Analysis Note

Evaluated by Immunohistochemistry in rat skeletal muscle tissue.

Immunohistochemistry Analysis: A 1:250 dilution of this antibody detected Obscurin in rat skeletal muscle tissue.

Other Notes

Concentration: Please refer to lot specific datasheet.


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存储类别

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable



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