Anti-Troponin T (Cardiac Muscle) Antibody, clone 9C2.1

Troponin T, cardiac muscle (UniProt P45379; also known as Cardiac muscle troponin T, cTnT, TnTc) is encoded by the TNNT2 (also known as CMD1D, CMH2, RCM3) gene (Gene ID 7139) in human. Troponin is a complex composed of three regulatory proteins, troponin C, troponin I, and troponin T). Troponin is found in both skeletal muscle and cardiac muscle where it plays an integral role in muscle contraction. Troponin T (TnT) is the component that binds tropomyosin, while troponin I (TnI) binds actin in thin myofilaments to hold the actin-tropomyosin complex in place. Three tissue TnT types exist, the slow skeletal troponin T1 encoded by the TNNT1 gene, the cardiac troponin T2 encoded by the TNNT2 gene, and the fast skeletal troponin T3 encoded by the TNNT3 gene.

Expected to react with human troponin T spliced isoforms 1-8 and 10, as well as all four of mouse troponin T spliced isoforms (Embryonic, A1, A2, and A3B isoforms; UniProt P50752) and both of rat troponin T spliced isoforms (Embryonic and Adult isoforms; UniProt P50753)

Epitope: Internal.

Recombinant human cardiac muscle troponin T internal fragment.

Detect Troponin T using this Anti-Troponin T (Cardiac Muscle) Antibody, clone 9C2.1 validated for use in Western Blotting, Immunohistochemistry.

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Troponin T (Cardiac Muscle) in rat cardiac muscle tissue.

Research Category
Cell Structure

Research Sub Category
Cytoskeleton

Evaluated by Western Blotting in mouse heart tissue lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected Troponin T (Cardiac Muscle) in 10 µg of mouse heart tissue lysate.

~36 kDa observed. 35.8 kDa (isoform Embryonic), 35.0 kDa (isoform A1), 34.5 kDa (isoform A2), and 34.2 kDa (Isoform A3B) of mouse cardiac muscle troponin T spliced isoforms calculated.

Protein G Purified

Format: Purified

Purified mouse monoclonal IgG2bκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Concentration: Please refer to lot specific datasheet.

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