MABT400
抗-润滑素/蛋白聚糖4，克隆5C11抗体
clone 5C11, from mouse
别名:
Proteoglycan 4, Lubricin, Megakaryocyte-stimulating factor, Superficial zone proteoglycan, Proteoglycan 4 C-terminal part
产品名称
抗-润滑素/蛋白聚糖4，克隆5C11抗体, clone 5C11, from mouse
biological source
mouse
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
5C11, monoclonal
species reactivity
canine, bovine, goat, rat, porcine, human, guinea pig
technique(s)
immunohistochemistry: suitable (paraffin)
western blot: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... PRG4(10216)
Analysis Note
通过蛋白质印迹法对润滑素纯化蛋白进行评价。
蛋白质印迹分析：1.0 µg/mL该抗体检测到10 µg纯化的润滑素/蛋白聚糖4蛋白。
蛋白质印迹分析：1.0 µg/mL该抗体检测到10 µg纯化的润滑素/蛋白聚糖4蛋白。
Application
免疫组织化学分析：一个代表性批次以1:1,000稀释度在人肝脏和人心脏组织中检测到润滑素/蛋白聚糖4。
蛋白质印迹分析：一个代表性批次检测到纯化人滑膜润滑素，以及从骨关节炎(OA)、屈曲指关节病-髋内翻-心包炎综合征(CACP)患者或从牛、猪、山羊、犬、大鼠和豚鼠中回收的滑液中检测到润滑素（Ai，M.，et al.（2015）.PLoS One.10（2）:e0116237）。
蛋白质印迹分析：在还原和非还原条件下，一个代表性批次检测到来自小牛和牛的纯化牛滑膜润滑素（Schmidt，T.A.，et al.（2009）.Biochim Biophys Acta.1790（5）:375-384）。
蛋白质印迹分析：一个代表性批次检测到纯化人滑膜润滑素，以及从骨关节炎(OA)、屈曲指关节病-髋内翻-心包炎综合征(CACP)患者或从牛、猪、山羊、犬、大鼠和豚鼠中回收的滑液中检测到润滑素（Ai，M.，et al.（2015）.PLoS One.10（2）:e0116237）。
蛋白质印迹分析：在还原和非还原条件下，一个代表性批次检测到来自小牛和牛的纯化牛滑膜润滑素（Schmidt，T.A.，et al.（2009）.Biochim Biophys Acta.1790（5）:375-384）。
经验证，该抗润滑素/蛋白聚糖4（克隆5C11）抗体可用于蛋白质印迹法和免疫组织化学（石蜡）检测润滑素/蛋白聚糖4。
General description
称为润滑素/蛋白聚糖4的蛋白质是关节中对关节功能至关重要的重要分泌蛋白，因此也是关节疾病研究的重点。润滑素/蛋白聚糖4在边界润滑中起作用，并通过控制滑膜细胞的滑膜生长和抑制细胞粘附到软骨表面来防止软骨从滑液中沉积。在发育过程中，润滑素/蛋白聚糖4还充当造血和内皮细胞谱系的生长因子，并且润滑素/蛋白聚糖4对于眼睛等组织中适当的屏障和润滑发展似乎很重要。润滑素/蛋白聚糖4在关节和关节边界附近高表达，但也可以在脑、心脏、肺、肾和其他组织中发现。润滑素/蛋白聚糖4具有几种亚型，它们的细胞和组织表达不同。亚型C和D是表达最广泛的形式。最后，由于其对表面的影响，正在组织工程和人工软骨开发中检查润滑素/蛋白聚糖4作为关节疾病的治疗方法。
观测分子量〜220 kDa。该蛋白质的计算分子量为151 kDa，在145 kDa左右观察到许多亚型，并且由于糖基化而可能更高。
Immunogen
对应于人润滑素/蛋白聚糖4的重组蛋白。
Other Notes
浓度：请参考特定批次的数据表。
Physical form
形式：纯化
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存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Patrick Weber et al.
Advanced healthcare materials, e2401623-e2401623 (2024-07-15)
Osteoarthritis is a degenerative joint disease that is associated with decreased synovial fluid viscosity and increased cartilage friction. Though viscosupplements are available for decades, their clinical efficacy is limited and there is ample need for more effective joint lubricants. This
Joseph A M Steele et al.
Biomaterials, 286, 121548-121548 (2022-05-20)
Articular cartilage is comprised of zones that vary in architecture, extracellular matrix composition, and mechanical properties. Here, we designed and engineered a porous zonal microstructured scaffold from a single biocompatible polymer (poly [ϵ-caprolactone]) using multiple fabrication strategies: electrospinning, spherical porogen
Taryn E Ludwig et al.
BMC musculoskeletal disorders, 16, 386-386 (2015-12-17)
Proteoglycan 4 (PRG4) and hyaluronan (HA) are key synovial fluid constituents that contribute synergistically to cartilage boundary lubrication; however, the effects of their concentrations as well as their structure, both of which can be altered in osteoarthritis, on this functional
A single-cell comparison of adult and fetal human epicardium defines the age-associated changes in epicardial activity.
Knight-Schrijver, et al.
Nature cardiovascular research, 1, 1215-1229 (2023)
Dongrim Seol et al.
Human gene therapy, 33(9-10), 529-540 (2021-10-07)
Lubricin, a glycoprotein encoded by the proteoglycan 4 (PRG4) gene, is an essential boundary lubricant that reduces friction between articular cartilage surfaces. The loss of lubricin subsequent to joint injury plays a role in the pathogenesis of posttraumatic osteoarthritis. In
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