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Merck
CN

MABT493

Anti-IL-34, clone 1D12 Antibody

ascites fluid, clone 1D12, from mouse

别名:

Interleukin-34, IL-34

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关于此项目

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
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产品名称

Anti-IL-34, clone 1D12 Antibody, ascites fluid, clone 1D12, from mouse

biological source

mouse

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

1D12, monoclonal

species reactivity

human

technique(s)

flow cytometry: suitable
immunofluorescence: suitable

isotype

IgG1

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Quality Level

Gene Information

human ... IL34(146433)

Preparation Note

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Flow Cytometry Analysis: A 1:200-400 dilution from a representative lot detected IL-34 in K562 cells.

Application

Anti-IL-34, clone 1D12 Antibody is an antibody against IL-34 for use in flow cytometry & immunofluorescence.
Immunofluorescence Analysis: A 1:200-1,000 dilution from a representative lot detected IL-34 in U251 cells.
Research Category
Cell Structure
Research Sub Category
Cytoskeleton

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

27 kDa calculated
Intereukin 34, also known as IL-34 or IL34 is a cytokine that promotes the differentiation and viability of moncytes and macrophages. IL-34 stimulate the phosphorylation of MAPL1/ERK2/ERK2 and MAPK3/ERK1. IL-34 is a ligand for colony-stimulating factor CSF1R. IL-34 is predominantly expressed in spleen, and also detected in a range of other tissues including heart, liver, kidney, thymus, testis, ovary, small intestine, prostate and colon.

Immunogen

Purified recombinant fragment of human IL-34 expressed in E. Coli

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Mouse monoclonal IgG1 ascitic fluid containing up to 0.1% sodium azide.
Unpurified

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存储类别

12 - Non Combustible Liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable


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Cancer immunology, immunotherapy : CII, 72(4), 851-864 (2022-09-15)
Triple-negative breast cancer (TNBC) is an aggressive breast cancer subtype characterized by a lack of therapeutic targets. The paucity of effective treatment options motivated a number of studies to tackle this problem. Immunosuppressive cells infiltrated into the tumor microenvironment (TME)
Nanumi Han et al.
STAR protocols, 2(2), 100460-100460 (2021-04-27)
Previously, we identified a therapy-resistant role of IL-34 in an immune checkpoint blockade in murine models. To investigate whether a similar mechanism is applicable in human tumors as well, we used this protocol for the selection of IL-34-neutralizing antibody and

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