Anti-β-Actin (ACTB) Antibody

Actin is a 43 kDa highly conserved structural protein. There are three main actin isotypes (alpha, beta and gamma) which show >90% amino-acid (aa) homology between isotypes and >98% homology within members of a particular isotypic group. The majority of the isotype heterogeneity is located in the amino-terminal 30 aa. The different actin isotypes have been shown to behave very differently in vitro and in vivo. Recent studies describe differential subcellular localization of gamma-actin and isotype specific binding of actin associated proteins. Beta actin is one of the two nonmuscle cytoskeletal actins and is relatively stable and appears to remain at normal levels regardless of experimental treatment. It is generally used as an internal control for experiments.

A peptide corresponding to the N-terminus of beta-Actin.

Immunocytochemistry Analysis (ICC): A 1:200 dilution from a representative lot detected beta-Actin in A431 cells.

This Anti-beta-Actin Antibody, clone RM112 is validated for use in Western Blotting, Immunocytochemistry for the detection of beta-Actin.

Evaluated by Western Blotting in A431 cell lysate.

Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected beta-Actin in A431 cell lysate.

~45 kDa observed. Uncharacterized bands may be observed in some lysate(s).

Format: Purified

Concentration: Please refer to lot specific datasheet.