跳转至内容
Merck
CN

MIT1000

线粒体/胞质溶胶分离试剂盒

This Mitochondria/Cytosol Fractionation Kit provides reagents for quick & efficient isolation of intact mitochondria from cultured cells.

登录 查看组织和合同定价。

选择尺寸

关于此项目

UNSPSC Code:
12161503
NACRES:
NA.75
eCl@ss:
32161000

主要文件

查看所有文档
技术服务
需要帮助？我们经验丰富的科学家团队随时乐意为您服务。
让我们为您提供帮助
技术服务
需要帮助？我们经验丰富的科学家团队随时乐意为您服务。
让我们为您提供帮助

产品名称

线粒体/胞质溶胶分离试剂盒, This Mitochondria/Cytosol Fractionation Kit provides reagents for quick & efficient isolation of intact mitochondria from cultured cells.

species reactivity (predicted by homology)

all

manufacturer/tradename

Chemicon®

technique(s)

cell culture | mammalian: suitable

application(s)

sample preparation

shipped in

dry ice

Quality Level

100

Application

研究类别
细胞凋亡 & 癌症

Disclaimer

除非我们的目录或产品随附的其他公司文件中另有说明，否则我们的产品预期仅用于研究用途，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或对人类或动物的任何类型的消费或应用。

General description

线粒体，有时被描述为细胞的“发电厂”，是真核细胞中大部分能量产生发生的场所。大多数三磷酸腺苷(ATP)的合成发生在活细胞中发现的这些双膜细胞器中。线粒体产生ATP是通过呼吸过程完成的，呼吸过程使用氧气产生能量。这是利用食物能量制造ATP的一个非常高效的过程。除了提供细胞能量外，线粒体还参与一系列其他过程，例如信号传导、细胞分化、细胞死亡以及细胞周期和细胞生长的控制。线粒体还调节关键的细胞凋亡信号通路。细胞中线粒体的数量因生物体和组织类型而异。许多细胞只有几个线粒体，而其他细胞则可以包含数千个。

Millipore’s线粒体/胞质分离试剂盒提供了从培养细胞中快速有效地分离完整线粒体的试剂。该试剂盒可通过使用方便的台式微量离心机分离线粒体，并可用于将富集的线粒体级分（重膜级分）与胞质级分（轻膜级分）分离。这种分离对于通过蛋白质印迹或ELISA研究两个部分之间的细胞凋亡和信号通路是有用的。

该试剂盒包含足以分析50个样品的材料。

Other Notes

等渗线粒体缓冲液（CS204255）: 50 mL

线粒体裂解缓冲液（CS204257）: 5 mL

蛋白酶抑制剂混合物（CS204253）: 1 mL

抗Bcl2（05-729-25UG）: 25 μg

抗GAPDH（CS204254）: 25 μg

Preparation Note

自接收之日起，所有组件应在-20°C下储存长达一年。

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

pictograms

Exclamation mark
GHS07

signalword

Warning

Hazard Classifications

Aquatic Chronic 3 - Eye Irrit. 2 - Skin Irrit. 2

存储类别

10 - Combustible liquids

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

已有该产品？

在文件库中查找您最近购买产品的文档。

访问文档库

Hongmei Li et al.
Nature cell biology, 15(7), 773-785 (2013-06-25)
Following exocytosis, the rate of recovery of neurotransmitter release is determined by vesicle retrieval from the plasma membrane and by recruitment of vesicles from reserve pools within the synapse, which is dependent on mitochondrial ATP. The anti-apoptotic Bcl-2 family protein
Jinxin Pan et al.
Central-European journal of immunology, 47(1), 20-29 (2022-05-24)
The protective activity of scopoletin (SPT) against glucose-induced cataract has been attributed to attenuation of aldose reductase activity and oxidative stress in a rat model. The present investigation was aimed to study the protective effect and mechanism of SPT in
Simon C Baker et al.
The American journal of pathology, 186(5), 1267-1277 (2016-03-24)
Recreational abuse of ketamine has been associated with the emergence of a new bladder pain syndrome, ketamine-induced cystitis, characterized by chronic inflammation and urothelial ulceration. We investigated the direct effects of ketamine on normal human urothelium maintained in organ culture
Turki Y Alhazzazi et al.
Anticancer research, 36(1), 49-60 (2016-01-02)
The survival rate of patients with head and neck squamous cell carcinoma (HNSCC) stands at approximately 50% and this has not improved in decades. This study developed a novel sirtuin-3 (SIRT3) inhibitor (LC-0296) and examined its role in altering HNSCC
Yoshimi Endo Greer et al.
Oncotarget, 9(26), 18454-18479 (2018-05-03)
We report a novel mechanism of action of ONC201 as a mitochondria-targeting drug in cancer cells. ONC201 was originally identified as a small molecule that induces transcription of TNF-related apoptosis-inducing ligand (TRAIL) and subsequently kills cancer cells by activating TRAIL
查看所有相关文献

相关内容

安定したタンパク質抽出と効率的なサンプル調整に　Buster & Protein Extraction Kit シリーズとタンパク質調整関連製品
Advancing cancer research: From hallmarks & biomarkers to tumor microenvironment progression

"Recognizing both the tremendous opportunities and the challenges facing cancer research, we are dedicated to developing and refining tools and technologies for the study of cancer. With our comprehensive portfolio, including the Upstate®, Chemicon®, and Calbiochem® brands of reagents and antibodies, researchers can count on dependable, high quality solutions for analyzing all the hallmarks of cancer."

我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系客户支持