Microcon^® DNA快速流动离心过滤器

Microcon® Centrifugal Filters simply and efficiently concentrate and desalt solutions of DNA, RNA, protein or other macromolecules, using any centrifuge that can accept 1.5 mL tubes.

The new dual cycle EtO-treated Microcon® DNA Fast Flow PCR Grade devices enable centrifugal concentration at a similar rate as the Microcon® DNA Fast Flow devices, but the EtO treatment greatly reduces the risk that no uncontrolled contaminating nucleic acids enter the analysis process. Both Microcon® devices exhibit faster flow rate (volume filtered per minute) compared to competitor centrifugal products.

Maximum recovery of pure intact genomic DNA from crude biological samples is the critical first step in any forensic analysis workflow. Typically, the process involves cell disruption, chemical or ion exchange-based extraction of the nucleic acid fraction and concentration of the purified DNA sample. While ethanol precipitation is commonly used for DNA concentration in research settings, an effective alternative is ultrafiltration via small spin columns such as the Microcon® DNA Fast Flow device. However, one overriding concern in the field of genetic testing is consumable product contamination with exogenous DNA during their assembly and packaging. To minimize this potential, a dual cycle ethylene oxide (EtO) treatment is employed to disrupt or fragment amplifiable DNA, thereby reducing the risk of false positives or uninterpretable results. To meet this concern, EMD Millipore has released the Microcon® DNA Fast Flow PCR Grade filter, an EtO-treated form of the parent device that has been shown here to match the original Microcon® device in performance.