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文件

MSIPS4510

Multiscreen^® 96孔板，疏水PVDF薄膜

Name: Multiscreen<SUP>®</SUP> 96孔板，疏水PVDF薄膜
Brand: MM

pore size 0.45 μm, sterile

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eCl@ss:

32039006

NACRES:

NB.22

物料

PVDF membrane (hydrophobic)
acrylic
flat bottom wells

质量水平

400

描述

Sterile, clear 96-well filter plate with 0.45 um pore size Hydrophobic PVDF membrane for ELISPOT Assays. Comes in a pack of 10.

无菌性

sterile

产品线

MultiScreen^®

特点

hydrophobic
lid

制造商/商品名称

MultiScreen^®

参数

50-250 μL sample volume (per well)

技术

ELISPOT: suitable

过滤面积

0.28 cm²

板尺寸

96 wells

孔最大体积

300 μL

工作体积

50-250 μL

基质

Immobilon^®-P

孔径

0.45 μm pore size

输入

sample type hematopoietic stem cell(s)
sample type pancreatic stem cell(s)
sample type: mouse embryonic stem cell(s)
sample type epithelial cells
sample type induced pluripotent stem cell(s)
sample type mesenchymal stem cell(s)
sample type: human embryonic stem cell(s)
sample type neural stem cell(s)
sample type cardiac stem cell(s)

吸附类型

high binding surface

运输

ambient

一般描述

无论您’的应用是什么，密理博都为满足您的具体应用需求开发出适用的过滤板。MultiScreen过滤板长期以来一直都是促进药物研发和生命科学研究的可靠工具。

HTS过滤板（以MS开头的板目录号）
- MultiScreenHTS过滤板专为自动工作站的大呑吐量应用而开发。坚固的侧壁更易于使用，充裕的表面提供了贴条形码标签的地方。每个板孔都单独密封，防止培养基互扰，而且下排水是可以取下的，从那里可以接触到滤膜。过滤板有96孔和384孔平台，具有广泛的膜和塑料选择。

- Millipore’s的新型MultiScreen HTS+大流量过滤板设计减轻了非特异性结合，减小了背景噪音和信号强度的差异，尤其是对生化筛选测定。

传统过滤板（以MS开头的板目录号）
- 传统的多孔设计对要求将滤膜浸入测定皿的许多应用特别有价值。易于拆卸的下排水管和独特的板设计是Elispot、PAMPA、细胞筛选（迁移、侵袭或趋化性）或topcount检测用户等分析的理想选择。

配件 – MultiScreenHTS真空多联装置
- MultiScreenHTS真空多联装置专门设计用于与MultiScreenHTS过滤板配合使用。这一组合提供了优化的过滤特征以及无缝自动化整合。

Millicell测定板
- 除了传统的测定板，过滤板还有配装细胞培养皿的型号，用于培养和测定悬浮的和粘附的细胞系。在滤板上生长的细胞比在传统塑料基质上生长的细胞分化更好，从而产生更多的体内样结果。该滤板/托盘组合可用于多种细胞测定，包括转运、毒性和筛选测定。

滤器编码：知识产权

应用

无菌、无色96孔过滤板，配有孔径为0.45 um的疏水性PDVF滤膜，用于ELISPOT测定。每包10个。

研究类别
测定

细胞培养

外形

色码：透明

其他说明

自动化兼容性：是

法律信息

Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany

MULTISCREEN is a registered trademark of Merck KGaA, Darmstadt, Germany

分析证书(COA)

输入产品批号来搜索分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

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示例

T1503

货号

25G

包装规格/数量

其它示例：

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

输入内容 1.000309185)

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如何查找COA批号

批号可以在产品标签上"批“ （Lot或Batch）字后面找到。

Aldrich 产品

如果您查询到的批号为 TO09019TO 等，请输入去除前两位字母的批号：09019TO。
如果您查询到的批号含有填充代码（例如05427ES-021），请输入去除填充代码-021的批号：05427ES。
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未找到您寻找的产品？

部分情况下，可能未在线提供COA。如果搜索不到COA，可在线索取。

索取COA

Critical parameters in blood processing for T-cell assays: validation on ELISpot and tetramer platforms.

Afonso, Georgia, et al.

Journal of Immunological Methods, 359, 28-36 (2010)

T cell receptor cross-reactivity between gliadin and bacterial peptides in celiac disease.

Jan Petersen et al.

Nature structural & molecular biology, 27(1), 49-61 (2019-12-25)

The human leukocyte antigen (HLA) locus is strongly associated with T cell-mediated autoimmune disorders. HLA-DQ2.5-mediated celiac disease (CeD) is triggered by the ingestion of gluten, although the relative roles of genetic and environmental risk factors in CeD is unclear. Here

Antibody Responses to Immunization With HCV Envelope Glycoproteins as a Baseline for B-Cell-Based Vaccine Development.

Fang Chen et al.

Gastroenterology, 158(4), 1058-1071 (2019-12-07)

We investigated antibody responses to hepatitis C virus (HCV) antigens E1 and E2 and the relevance of animal models for vaccine development. We compared antibody responses to vaccination with recombinant E1E2 complex in healthy volunteers, non-human primates (NHPs), and mice.

One dose of a porcine circovirus 2 subunit vaccine induces humoral and cell-mediated immunity and protects against porcine circovirus-associated disease under field conditions.

Martelli, Paolo, et al.

Veterinary Microbiology (2010)

Evaluation of the immune response induced by intradermal vaccination by using a needle-less system in comparison with the intramuscular route in conventional pigs.

Ferrari, L, et al.

Research in Veterinary Science (2010)

查看所有相关文献

商品

A Highly Sensitive IFN-γ ELISpot Assay to Quantify Cellular Immune Responses to Previous Viral Infection

This study presents an example of a typical ELISpot assay for quantifying the number of pre-existing, antigen-reactive T cells from peripheral blood mononuclear cell (PBMC) samples obtained from donors previously infected with cytomegalovirus (CMV).

The ELISpot Assay Enables Functional Analysis of Cellular Immunology

The enzyme-linked immunosorbent spot (ELISpot ) assay enables visualization of multiple secretory products from a single responding cell. The ELISpot provides both qualitative (type of immune protein) and quantitative (number of responding cells) information.

实验方案

IFN-γ ELISpot Assays on MultiScreen® IP

The ELISpot (Enzyme Linked Immuno-Spot) assay provides an effective method of measuring the antibody or cytokine production of immune cells on the single cell level.

Guidelines for Bioassays on MultiScreen Filter Plates: Protein Binding and Cell Loading

Guidelines for various bioassays on the MultiScreen system, which makes it possible to carry out an assay from sample incubation to scintillation counting in a 96-well plate.

文件

Multiscreen® 96孔板，疏水PVDF薄膜