Merck
CN
所有图片(1)

文件

MSIPS4W10

Millipore

Multiscreen® 96孔板，疏水PVDF薄膜

pore size 0.45 μm, sterile

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所有图片(1)
eCl@ss:
32039006
NACRES:
NB.22

物料

PVDF membrane (hydrophobic)
acrylic
flat bottom wells

质量水平

400

描述

Sterile, white 96-well filter plate with 0.45 um pore size Hydrophobic PVDF membrane for ELISPOT Assays. Comes in a pack of 10.

无菌性

sterile

产品线

MultiScreen®

特点

hydrophobic
lid

制造商/商品名称

MultiScreen®

参数

50-250 μL sample volume (per well)

技术

ELISPOT: suitable

过滤面积

0.28 cm2

板尺寸

96 wells

孔最大体积

300 μL

工作体积

50-250 μL

基质

Immobilon®-P

孔径

0.45 μm pore size

吸附类型

high binding surface

运输

ambient

相关类别

一般描述

聚偏氟乙烯(PVDF)滤膜板胜过混合纤维素酯膜，对捕获抗体的结合能力略有提高，斑点检测性能优异，尤为适合荧光法应用。

应用

MultiScreen® HTS IP过滤板（0.45 μm，白色，无菌）已用于下列细胞的酶联免疫斑点法(ELISpot)分析：
  • 产双链DNA的浆细胞
  • 记忆B细胞和骨髓长寿浆细胞
  • 引流淋巴结和脾脏细胞

法律信息

Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany
MULTISCREEN is a registered trademark of Merck KGaA, Darmstadt, Germany

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Katrin Klocke et al.
Immunology, 152(1), 125-137 (2017-05-13)
Cytotoxic T-lymphocyte antigen 4 (CTLA-4) -mediated regulation of already tolerized autoreactive T cells is critical for understanding autoimmune responses. Although defects in CTLA-4 contribute to abnormal FOXP3+ regulatory T (Treg) cell function in rheumatoid arthritis, its role in autoreactive T
Jing Zhu et al.
Journal of immunology (Baltimore, Md. : 1950), 204(5), 1091-1100 (2020-01-29)
Almost a decade has passed since the approval of belimumab, an mAb directed against B lymphocyte stimulation and the first targeted therapy approved for systemic lupus erythematous (SLE) in over 50 y. Although well tolerated, the efficacy of belimumab remains
Leah E Cole et al.
Vaccines, 8(4) (2020-12-06)
While both whole-cell (wP) and acellular pertussis (aP) vaccines have been highly effective at reducing the global pertussis disease burden, there are concerns that compared to wP vaccination, the immune responses to aP vaccination may wane more rapidly. To gain
Mubeen M Mosaheb et al.
Nature communications, 11(1), 524-524 (2020-01-29)
Viruses naturally engage innate immunity, induce antigen presentation, and mediate CD8 T cell priming against foreign antigens. Polioviruses can provide a context optimal for generating antigen-specific CD8 T cells, as they have natural tropism for dendritic cells, preeminent inducers of

商品

The ELISpot Assay Enables Functional Analysis of Cellular Immunology

The enzyme-linked immunosorbent spot (ELISpot ) assay enables visualization of multiple secretory products from a single responding cell. The ELISpot provides both qualitative (type of immune protein) and quantitative (number of responding cells) information.

实验方案

IFN-γ ELISpot Assays on MultiScreen® IP

The ELISpot (Enzyme Linked Immuno-Spot) assay provides an effective method of measuring the antibody or cytokine production of immune cells on the single cell level.

Guidelines for Bioassays on MultiScreen Filter Plates: Protein Binding and Cell Loading

Guidelines for various bioassays on the MultiScreen system, which makes it possible to carry out an assay from sample incubation to scintillation counting in a 96-well plate.

相关内容

Chemical Compatibility Guide for MultiScreen® Filter Plates

This provides information about the chemical compatibility of MultiScreen Filter Plates with different reagents.

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