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Merck
CN

MSIPS4W10

Multiscreen® 96孔板，疏水PVDF薄膜

pore size 0.45 μm, sterile

别名:

PVDF 96孔板, 疏水性96孔板

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UNSPSC代码:
41104923
eCl@ss:
32039006
NACRES:
NB.22

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物料

Durapore PVDF membrane
flat bottom wells
white acrylic plate

质量水平

400

描述

Sterile, white 96-well filter plate with 0.45 um pore size Hydrophobic PVDF membrane for ELISPOT Assays. Comes in a pack of 10.

无菌性

sterile

用途

Automated

特点

hydrophobic
lid

包装

pkg of 10 plates

制造商/商品名称

MultiScreen®

技术

ELISPOT: suitable
cell based assay: suitable
dot blot: suitable

过滤面积

0.28 cm2

板尺寸

96 wells

孔最大体积

300 μL

工作体积

50-250 μL

基质

Immobilon®-P

孔径

0.45 μm pore size

吸附类型

high binding surface

相容性

centrifuge & manifold

运输

ambient

相关类别

一般描述

聚偏氟乙烯(PVDF)滤膜板胜过混合纤维素酯膜，对捕获抗体的结合能力略有提高，斑点检测性能优异，尤为适合荧光法应用。

应用

MultiScreen® HTS IP过滤板（0.45 μm，白色，无菌）已用于下列细胞的酶联免疫斑点法(ELISpot)分析：
  • 产双链DNA的浆细胞
  • 记忆B细胞和骨髓长寿浆细胞
  • 引流淋巴结和脾脏细胞

法律信息

Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany
MULTISCREEN is a registered trademark of Merck KGaA, Darmstadt, Germany

分析证书(COA)

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Katrin Klocke et al.
Immunology, 152(1), 125-137 (2017-05-13)
Cytotoxic T-lymphocyte antigen 4 (CTLA-4) -mediated regulation of already tolerized autoreactive T cells is critical for understanding autoimmune responses. Although defects in CTLA-4 contribute to abnormal FOXP3+ regulatory T (Treg) cell function in rheumatoid arthritis, its role in autoreactive T
Leah E Cole et al.
Vaccines, 8(4) (2020-12-06)
While both whole-cell (wP) and acellular pertussis (aP) vaccines have been highly effective at reducing the global pertussis disease burden, there are concerns that compared to wP vaccination, the immune responses to aP vaccination may wane more rapidly. To gain
Mubeen M Mosaheb et al.
Nature communications, 11(1), 524-524 (2020-01-29)
Viruses naturally engage innate immunity, induce antigen presentation, and mediate CD8 T cell priming against foreign antigens. Polioviruses can provide a context optimal for generating antigen-specific CD8 T cells, as they have natural tropism for dendritic cells, preeminent inducers of
Jing Zhu et al.
Journal of immunology (Baltimore, Md. : 1950), 204(5), 1091-1100 (2020-01-29)
Almost a decade has passed since the approval of belimumab, an mAb directed against B lymphocyte stimulation and the first targeted therapy approved for systemic lupus erythematous (SLE) in over 50 y. Although well tolerated, the efficacy of belimumab remains

商品

ELISpot Assay: Functional Cellular Immunology

ELISpot assay provides qualitative and quantitative information on immune responses, visualizing multiple secretory products from single responding cells.

实验方案

IFN-γ ELISpot Assays on MultiScreen® IP

The ELISpot (Enzyme Linked Immuno-Spot) assay provides an effective method of measuring the antibody or cytokine production of immune cells on the single cell level.

Guidelines for Bioassays on MultiScreen Filter Plates: Protein Binding and Cell Loading

Guidelines for various bioassays on the MultiScreen system, which makes it possible to carry out an assay from sample incubation to scintillation counting in a 96-well plate.

相关内容

Chemical Compatibility Guide for MultiScreen® Filter Plates

This provides information about the chemical compatibility of MultiScreen Filter Plates with different reagents.

MultiScreen®Filter Plates Poster

MultiScreen® Filter Plates

MultiScreen®HTS and MultiScreen®HTS+ Hi Flow Assay Systems
IFN-γ ELISpot Assays on MultiScreen® IP

The ELISpot (Enzyme Linked Immuno-Spot) assay provides an effective method of measuring the antibody or cytokine production of immune cells on the single cell level. The popularity of this assay has seen resurgence in recent years as researchers attempt to gain a better understanding of immune responses in a variety of applications. The following protocol is an example of a typical ELISpot assay for quantifying the number cells producing interferon-gamma (IFN-γ) in response to antigen or non-specific activation using phytohemagglutinin (PHA). It may be optimized as necessary for other applications.

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