产品名称
抗-锚蛋白G小鼠单克隆抗体(463), liquid, ≥95% (SDS-PAGE), clone 463
biological source
mouse
antibody form
purified antibody
antibody product type
primary antibodies
clone
463, monoclonal
assay
≥95% (SDS-PAGE)
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity
rat, mouse, human, canine
manufacturer/tradename
Calbiochem®
storage condition
do not freeze
dilution
(Frozen Sections (1-2 µg/mL)
Immunoblotting (2-5 µg/mL)
Immunofluorescence (1-2 µg/mL))
isotype
IgG1
shipped in
wet ice
storage temp.
2-8°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... ANK3(288)
General description
抗锚蛋白G,小鼠单克隆,克隆463,识别脑提取物中的〜190 kDa锚蛋白G蛋白。它已被验证可用于蛋白质印迹、免疫荧光和冷冻切片。
识别脑提取物中的~190 kDa锚蛋白G。与锚蛋白G的所有剪接形式反应。抗体靶基因符号: ANK3 目标同义词: 2900054D09RIK、AI314020、AnkG、锚蛋白-3、锚蛋白-G、FLJ45464、MGC14049 Entrez基因名称:锚蛋白3、郎飞氏结(锚蛋白G) Hu Entrez ID:288(相关抗体:AP1034、PC523、PC522、AP1057) Mu Entrez ID:11735 大鼠Entrez ID:361833
通过使用指定的免疫原免疫BALB/c小鼠并将脾细胞与P3X63/Ag 8.653小鼠骨髓瘤细胞融合而产生的纯化小鼠单克隆抗体。识别~190 kDa的锚蛋白G亚型。
Analysis Note
阳性对照
小鼠或大鼠脑提取物或无髓鞘轴突
小鼠或大鼠脑提取物或无髓鞘轴突
Application

冷冻切片(1-2 g/mL)
免疫印迹(2-5 g/mL)
免疫荧光(1-2 g/mL)
Disclaimer
毒性:标准处理(A)
Immunogen
一种与来自人锚蛋白G的血影蛋白结合域的氨基酸相对应的合成肽
人
Other Notes
Davis, J.Q., et al. 1995.Mol.Biol. Cell6, 177a, supplement.
Kunimoto, M., et al. 1995.FEBS Lett.357, 217.
Lambert, S., et al. 1995.Mol.Biol. Cell6, 98a supplement.
Bennett, V. and Gilligan, D.M.1993.Ann.Rev. Cell Biol.9, 27.
Kunimoto, M., et al. 1991.J. Cell Biol.115, 1319.
Otto, E., et al. 1991.J. Cell Biol.114, 241.
Davis, J., and Bennett, V. 1984.J. Biol. Chem.259, 13550.
Kunimoto, M., et al. 1995.FEBS Lett.357, 217.
Lambert, S., et al. 1995.Mol.Biol. Cell6, 98a supplement.
Bennett, V. and Gilligan, D.M.1993.Ann.Rev. Cell Biol.9, 27.
Kunimoto, M., et al. 1991.J. Cell Biol.115, 1319.
Otto, E., et al. 1991.J. Cell Biol.114, 241.
Davis, J., and Bennett, V. 1984.J. Biol. Chem.259, 13550.
锚蛋白G是轴突起始节段、Ranvier结节和上皮细胞-细胞接触部位的良好免疫组织化学标志物。在单个系统中,应对抗体进行滴定以获得最佳结果。
Packaging
批次特定浓度请参见小瓶标签。
Physical form
在50 mM磷酸钠缓冲液,0.2%明胶,pH 7.5中。
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
10 - Combustible liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
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