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Merck
CN

NS170

Multi Species Phosphorylated Neurofilament ELISA Kit

measures and quantifies pNF-H levels in serum or plasma

别名:

200 kDa neurofilament protein, Phosphorylated Neurofilament heavy polypeptide, pNF-H

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关于此项目

UNSPSC Code:
12161503
eCl@ss:
32161000
NACRES:
NA.84
Species reactivity:
pig, feline, rat, human, bovine, mouse
Shipped in:
dry ice
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产品名称

磷酸化神经丝(pNF-H)夹心ELISA试剂盒, The Phosphorylated Neurofilament H (pNF-H) kit is a sandwich enzyme immunoassay (EIA), which measures pNF-H.

species reactivity

pig, feline, rat, human, bovine, mouse

manufacturer/tradename

Chemicon®

assay range

sensitivity: 0.0293-15 ng/mL
(Range of Detection), sensitivity: 0.0585 ng/mL

technique(s)

ELISA: suitable

NCBI accession no.

UniProt accession no.

application(s)

research use

detection method

colorimetric

shipped in

dry ice

Quality Level

Application

用于检测/量化:磷酸化神经丝(pNF-H)
研究类别
神经科学
磷酸化神经丝H(pNF-H)试剂盒是一种夹心酶免疫测定法(EIA),可测量pNF-H。

Packaging

96孔

Preparation Note

使用前,按照指示将试剂盒组件保持在2°-8°C和-20°C,直至失效日期。

1.清洗缓冲液-稀释后,将清洗液在2°-8°C下保存长达一个月或直至失效日期。

2.Tween® 20 - 收到试剂盒后在室温下储存。

3.Elisa试验缓冲液-根据需要等分,以避免重复冻融循环。解冻后,将Elisa分析缓冲液在2°-8°C下保存长达一个月,或直至失效日期。

4. 预先用鸡抗pNF-H多克隆抗体包被的免疫板-未使用的试剂条微孔可放回铝箔袋中,并在2°-8°C下储存长达1个月或直至失效日期。

Other Notes

鸡抗-pNF-H多克隆抗体预包被的免疫板:(部件号2006263)。一个预先包被有鸡抗pNF-H多克隆抗体的96孔免疫板,密封在铝箔袋中。

兔抗pNF-H多克隆抗体:(部件号2006919)。一瓶(100 μL)兔抗pNF-H多克隆抗体。

山羊抗兔碱性磷酸酶偶联抗体:(部件号2006917)。一瓶(10 μL)山羊抗兔碱性磷酸酶结合物。

牛pNF-H标准品:(部件号2006918)。一瓶(5.0 ng)牛pNF-H标准品。

20X TBS:(部件号2004913)。一瓶(50 mL)20X TBS。

20% Tween® 20:(部件号2004915)。一瓶(3 mL)20% 吐温-20。

Elisa检测稀释剂:(部件号2006921)一瓶(30 mL)Elisa检测稀释剂,含有0.05%Kathon作为防腐剂,以即用型形式提供。

pNPP缓冲液:(部件号2006261)。一瓶(12 mL)pNPP缓冲液,与50X p-NPP一起使用,以即用型形式提供。

50X pNPP:(部件号2006262)。一瓶(250 μL)50X pNPP(对硝基苯基磷酸酶)碱性磷酸酶底物。

板盖-两个板盖。

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
TWEEN is a registered trademark of Croda International PLC

Disclaimer

仅供研究使用。不用于诊断操作。

pictograms

Health hazardCorrosion

signalword

Danger

Hazard Classifications

Eye Dam. 1 - Repr. 2 - Skin Irrit. 2 - STOT RE 2 Oral

target_organs

Kidney,Liver,Blood

存储类别

10 - Combustible liquids

wgk

WGK 3

法规信息

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R Takano et al.
Neurology, 75(3), 208-216 (2010-07-21)
Loss of aquaporin 4 and glial fibrillary acidic protein (GFAP) with necrosis and demyelination is a prominent pathologic feature of neuromyelitis optica (NMO). However, the clinicopathologic significance of astrocytic damage and its relation with demyelination are unknown. To analyze clinical
J S Pachter et al.
Developmental biology, 103(1), 200-210 (1984-05-01)
The ontogenetic appearance of the individual triplet polypeptides that comprise mammalian neurofilaments was studied in the developing rat optic nerve. Triton-insoluble cytoskeletal preparations from the optic nerves of rats of postnatal ages 1 Day (P1), 6 days (P6), 10 days
Gerry Shaw et al.
Biochemical and biophysical research communications, 336(4), 1268-1277 (2005-09-24)
Several lines of reasoning suggest that the phosphorylated axonal form of the neurofilament subunit NF-H is likely to be released from damaged and diseased neurons in significant amounts. Detection of this protein in serum or CSF might therefore provide information
Kevin J Anderson et al.
Journal of neurotrauma, 25(9), 1079-1085 (2008-08-30)
The detection of neuron-specific proteins in blood might allow quantification of the degree of neuropathology in experimental and clinical contexts. We have been studying a novel blood biomarker of axonal injury, the heavily phosphorylated axonal form of the high molecular
P N Hoffman et al.
The Journal of cell biology, 66(2), 351-366 (1975-08-01)
This study of the slow component of axonal transport was aimed at two problems: the specific identification of polypeptides transported into the axon from the cell body, and the identification of structural polypeptides of the axoplasm. The axonal transport paradigm

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