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Merck
CN

OB05

Anti-Histidine-Tagged Protein Mouse mAb (13/45/31/2)

liquid, clone 13/45/31/2, Calbiochem®

别名:

Anti-His6

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关于此项目

NACRES:
NA.41
UNSPSC Code:
12352203
Clone:
13/45/31/2, monoclonal
Species reactivity:
-
Application:
Citations:
10
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biological source

mouse

antibody form

purified antibody

antibody product type

primary antibodies

clone

13/45/31/2, monoclonal

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity (predicted by homology)

all

manufacturer/tradename

Calbiochem®

storage condition

do not freeze

dilution

(ELISA (0.1-0.4 µg/mL)
Immunoblotting (1-4 µg/mL)
Immunofluorescence (1-4 µg/mL)
Immunoprecipitation (4 µg/mL))

isotype

IgG1

shipped in

wet ice

Quality Level

target post-translational modification

unmodified

General description

Anti-Histidine-Tagged Protein, mouse monoclonal, clone 13/45/31/2, specifically recognizes an epitage of 6 consecutive His residues. It is validated for use in ELISA, WB, IF & IP.
Purified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with Sp2/0 mouse myeloma cells. Recognizes a His•Tag sequence in native and recombinant proteins. Specifically recognizes an epitope of six consecutive His residues of both natural and recombinant sources.
Specifically recognizes an epitage of six consecutive His residues of both natural and recombinant sources.

Immunogen

Epitope: HHHHHH
a recombinant protein containing a His•Tag sequence

Application



ELISA (0.1-0.4 g/ml)
Immunoblotting (1-4 g/ml)
Immunofluorescence (1-4 g/ml)
Immunoprecipitation (4 g/ml)

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.

Analysis Note

Positive Control
Proteins containing a His•Tag sequence

Other Notes

Antibody should be titrated for optimal results in individual systems.
Zentgraf, H., et al. 1995. Nucl. Acids. Res.23, 3347.
Gu, J., et al. 1994. BioTechniques17, 257.
Sporeno, E., et al. 1994. J. Biol. Chem., 10991.
Sisk, W.P., et al. 1994. J. Virol.68, 766.
Lu, T., et al. 1993. Anal. Biochem.213, 318.
Garner, J., et al. 1992. Cell69, 833.
Hochuli, E., et al. 1987. J. Chromatogr.411, 177.

Legal Information

Manufactured by Dianova, GmbH, Germany.
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

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存储类别

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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Jia Chen et al.
Journal of virology, 94(21) (2020-08-28)
Both Epstein-Barr virus (EBV) and Kaposi's sarcoma-associated herpesvirus (KSHV) are human gammaherpesviruses and are important in a variety of malignancies. Eph family receptor tyrosine kinase A2 (EphA2) is a cellular receptor for KSHV and EBV. Previous studies identified five conserved
Larry J Anderson et al.
Frontiers in immunology, 14, 1332772-1332772 (2024-01-29)
Effective respiratory syncytial virus (RSV) vaccines have been developed and licensed for elderly adults and pregnant women but not yet for infants and young children. The RSV immune state of the young child, i.e., previously RSV infected or not, is
Samadhan J Jadhao et al.
Journal of medical virology, 93(6), 3439-3445 (2020-12-17)
Respiratory syncytial virus (RSV) infection is a major cause of respiratory tract disease in young children and throughout life. Infant infection is also associated with later respiratory morbidity including asthma. With a prospective birth cohort study of RSV and asthma
J Mario Isas et al.
Nature communications, 12(1), 4272-4272 (2021-07-15)
The first exon of the huntingtin protein (HTTex1) important in Huntington's disease (HD) can form cross-β fibrils of varying toxicity. We find that the difference between these fibrils is the degree of entanglement and dynamics of the C-terminal proline-rich domain
Maureen C Dolan et al.
Methods in molecular biology (Clifton, N.J.), 824, 65-105 (2011-12-14)
Protein-specific antibodies serve as critical tools for detection, quantification, and characterization of recombinant proteins. Perhaps the most important and widely used antibody-based procedures for recombinant protein applications are Western immunoblotting and enzyme-linked immunosorbent assays (ELISAs). These analyses require well-characterized, sensitive

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