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OP107

Anti-BRCA1 (Ab-4) Mouse mAb (SD118)

Name: Anti-BRCA1 (Ab-4) Mouse mAb (SD118)
Brand: MM

liquid, clone SD118, Calbiochem^®

别名:

Anti-Breast Cancer Susceptibility Protein

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关于此项目

NACRES:

NA.43

UNSPSC Code:

12352203

Clone:

SD118, monoclonal

Species reactivity:

human

Application:

—

Citations:

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属性

biological source

mouse

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

SD118, monoclonal

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity

human

manufacturer/tradename

Calbiochem^®

storage condition

do not freeze

dilution

(Frozen Sections (2 µg/mL)
Immunoblotting (2 µg/mL,
Immunofluorescence (2 µg/mL)
Immunoprecipitation (2 µg,
Paraffin Sections (not recommended))

isotype

IgG1

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

Gene Information

human ... BRCA1(672)

说明

General description

Anti-BRCA1 (Ab-4), mouse monoclonal, clone SD118, recognizes the ~220 kDa BRCA1 in HBL-100 and SK-BR3 cells. It is validated for WB, IF, IP, and for frozen sections.

Purified mouse monoclonal antibody generated by immunizing RBF/DnJ mice with the specified immunogen and fusing splenocytes with NS1 mouse myeloma cells (see application references). Recognizes the ~220 kDa BRCA1 protein.

Recognizes the ~220 kDa BRCA1 protein in HBL-100 and SK-BR3 cells. Sold under license of U.S. Patent 5,753,441 and 6,162,897.

Immunogen

Human

a recombinant polypeptide corresponding to amino acids 1005-1313 in exon 11 of human BRCA1

Application

Frozen Sections (2 µg/ml)

Immunoblotting (2 µg/ml, see application references)

Immunofluorescence (2 µg/ml)

Immunoprecipitation (2 µg, see application references)

Paraffin Sections (not recommended)

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.

Analysis Note

Positive Control
HBL-100 or SK-BR3 cells

Other Notes

Chen, J.J., et al. 1999. Cancer Res.59, 1752s.
Cressman, V.L., et al. 1999. Cell Growth Differ.10, 1.
Irminger-Finger, I., et al. 1999. Biol. Chem.380, 117.
Xu, X., et al. 1999. Nat. Genet.22, 37.
Wilson, C.A., et al. 1999. Nat. Genet.21, 236.
Andres, J.L., et al. 1998. Oncogene16, 2229.
Chen, J., et al. 1998. Mol. Cell2, 317.
Chen, P.L., et al. 1998. Proc. Natl. Acad. Sci. USA95, 5287.
Fan, S., et al. 1998. Int. J. Cancer77, 600.
Gowen, L.C., et al. 1998. Science281, 1009.
Hsu, L.C. and R.L. White. 1998. Proc. Natl. Acad. Sci. USA95, 12983.
Shen, S.X., et al. 1998. Oncogene17, 3115.
Tulchin, N., et al. 1998. Int. J. Oncol.13, 513.
Ludwig, T., et al. 1997. Genes Dev11, 1226.
Ruffner, H. and I.M. Verma. 1997. Proc. Natl. Acad. Sci. USA94, 7138.
Scully, R., et al. 1997. Cell90, 425.
Thakur, S., et al. 1997. Mol. Cell Biol.17, 444.
Wilson, C.A., et al. 1997. Oncogene14, 1.
Cannon-Albright, L.A. and M.H. Skolnick. 1996. Semin Oncol.23, 1.
Chen, Y., et al. 1996. Cancer Res.56, 3168.
Liu, C.Y., et al. 1996. Genes Dev.10, 1835.
Gudas, J.M., et al. 1996. Cell Growth Differ.7, 717.
Scully, R., et al. 1996. Science272, 123.
Vaughn, J.P., et al. 1996. Cell Growth Differ.7, 711.
Wilson, C.A. et al. 1996. Nat. Genet.13, 264.
Thompson, M.E., et al. 1995. Nat. Genet.9, 444.
Miki, Y., et al. 1994. Science266, 66.

Maximal BRCA1 levels are observed in subconfluent cell populations after serum stimulation. Highly specific BRCA1 antibody useful for blotting and IP of full length BRCA1 protein. Antibody should be titrated for optimal results in individual systems.

Legal Information

Sold under license of U.S. Patents 5,753,441 and 6,162,897.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

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存储类别

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

法规信息

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The in vivo dynamic organization of BRCA1-A complex proteins at DNA damage-induced nuclear foci.

Myth T S Mok et al.

Traffic (Copenhagen, Denmark), 13(6), 800-814 (2012-03-17)

The breast cancer associated gene 1 (BRCA1)-A protein complex assembles at DNA damage-induced nuclear foci to facilitate repair of double-stranded breaks. Here, we describe the first systematic comparison of the dynamics, copy number and organization of its core components at

Multifunctional transcription factor TFII-I is an activator of BRCA1 function.

M Tanikawa et al.

British journal of cancer, 104(8), 1349-1355 (2011-03-17)

The TFII-I is a multifunctional transcriptional factor known to bind specifically to several DNA sequence elements and to mediate growth factor signalling. A microdeletion at the chromosomal location 7q11.23 encoding TFII-I and the related family of transcription factors may result

DNA damage induced during mitosis undergoes DNA repair synthesis.

Veronica Gomez Godinez et al.

PloS one, 15(4), e0227849-e0227849 (2020-04-29)

Understanding the mitotic DNA damage response (DDR) is critical to our comprehension of cancer, premature aging and developmental disorders which are marked by DNA repair deficiencies. In this study we use a micro-focused laser to induce DNA damage in selected

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货号	GTIN
OP107-100UG	04055977224924