OP140
Anti-p53 (Ab-12) (Pantropic) Mouse mAb (DO-7)
liquid, clone DO-7, Calbiochem®
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关于此项目
NACRES:
NA.41
UNSPSC Code:
12352203
Clone:
DO-7, monoclonal
Species reactivity:
bovine, monkey, human
Application:
—
Citations:
11
biological source
mouse
antibody form
purified antibody
antibody product type
primary antibodies
clone
DO-7, monoclonal
form
liquid
does not contain
preservative
species reactivity
bovine, monkey, human
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, avoid repeated freeze/thaw cycles
dilution
(Frozen Sections (1-2 µg/mL)
Immunoblotting (1-2 µg/mL)
Immunofluorescence (0.5 µg/mL)
Immunoprecipitation (2 µg/mg lysate)
Paraffin Sections (1-2 µg/mL))
isotype
IgG2b
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... TP53(7157)
General description
Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen. Recognizes the ~53 kDa wild-type and mutant forms of p53.
Recognizes the ~53 kDa wild-type p53 and mutant p53 protein in SKBR-3 and MDA-231 cells and in colon carcinoma tissue.
This Anti-p53 (Ab-12) (Pantropic) Mouse mAb (DO-7) is validated for use in Frozen Sections, Immunoblotting, IF, IP, Paraffin Sections for the detection of p53 (Ab-12) (Pantropic).
Immunogen
Epitope: within amino acids 37-45 of p53
recombinant, human p53 expressed in E. coli
Application
Frozen Sections (1-2 g/ml)
Immunoblotting (1-2 g/ml)
Immunofluorescence (0.5 g/ml)
Immunoprecipitation (2 g/mg lysate)
Paraffin Sections (1-2 g/ml)
Packaging
Please refer to vial label for lot-specific concentration.
Analysis Note
Positive Control
SKBR-3 or MDA-231 cells or colon carcinoma tissue
SKBR-3 or MDA-231 cells or colon carcinoma tissue
Other Notes
Exhibits nuclear staining in tumor cells. Antibody should be titrated for optimal results in individual systems.
Greenblatt, M.S., et al. 1994. Cancer Res.54, 4855.
Lane, D.P. 1992. Nature358, 15.
Lane, D.P. 1992. Nature358, 15.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
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存储类别
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Jinichi Mori et al.
Neoplasia (New York, N.Y.), 19(3), 185-195 (2017-02-06)
p53 activation by cellular stresses induces the transcription of hundreds of its target genes. To elucidate the entire picture of its downstream pathway, we screened a cDNA microarray dataset of adriamycin-treated HCT116 p53-/- or p53+/+ cells and identified EPSIN 3
Franz Meitinger et al.
The Journal of cell biology, 214(2), 155-166 (2016-07-20)
In normal human cells, centrosome loss induced by centrinone-a specific centrosome duplication inhibitor-leads to irreversible, p53-dependent G1 arrest by an unknown mechanism. A genome-wide CRISPR/Cas9 screen for centrinone resistance identified genes encoding the p53-binding protein 53BP1, the deubiquitinase USP28, and
Jinichi Mori et al.
Cancer science, 107(3), 298-306 (2016-01-13)
In response to various cellular stresses, p53 is activated and inhibits malignant transformation through the transcriptional regulation of its target genes. However, the full picture of the p53 downstream pathway still remains to be elucidated. Here we identified cystatin C
Varalee Yodsurang et al.
Oncotarget, 8(34), 55790-55803 (2017-09-17)
p53 mutation is a marker of poor prognosis in breast cancers. To identify downstream targets of p53, we screened two transcriptome datasets, including cDNA microarrays of MCF10A breast epithelial cells with wild-type p53 or p53-null background, and RNA sequence analysis
Tomoyuki Koguchi et al.
International journal of oncology, 48(6), 2415-2424 (2016-04-02)
In response to various cellular stresses, p53 exerts its tumor suppressive effects such as apoptosis, cell cycle arrest, and senescence through the induction of its target genes. Recently, p53 was shown to control cellular homeostasis by regulating energy metabolism, glycolysis
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