biological source
mouse
Quality Segment
antibody form
purified antibody
antibody product type
primary antibodies
clone
DO-7, monoclonal
form
liquid
does not contain
preservative
species reactivity
bovine, monkey, human
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, avoid repeated freeze/thaw cycles
dilution
(Frozen Sections (1-2 µg/mL)
Immunoblotting (1-2 µg/mL)
Immunofluorescence (0.5 µg/mL)
Immunoprecipitation (2 µg/mg lysate)
Paraffin Sections (1-2 µg/mL))
isotype
IgG2b
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... TP53(7157)
General description
Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen. Recognizes the ~53 kDa wild-type and mutant forms of p53.
Recognizes the ~53 kDa wild-type p53 and mutant p53 protein in SKBR-3 and MDA-231 cells and in colon carcinoma tissue.
This Anti-p53 (Ab-12) (Pantropic) Mouse mAb (DO-7) is validated for use in Frozen Sections, Immunoblotting, IF, IP, Paraffin Sections for the detection of p53 (Ab-12) (Pantropic).
Immunogen
Epitope: within amino acids 37-45 of p53
recombinant, human p53 expressed in E. coli
Application
Frozen Sections (1-2 g/ml)
Immunoblotting (1-2 g/ml)
Immunofluorescence (0.5 g/ml)
Immunoprecipitation (2 g/mg lysate)
Paraffin Sections (1-2 g/ml)
Packaging
Please refer to vial label for lot-specific concentration.
Analysis Note
Positive Control
SKBR-3 or MDA-231 cells or colon carcinoma tissue
SKBR-3 or MDA-231 cells or colon carcinoma tissue
Other Notes
Exhibits nuclear staining in tumor cells. Antibody should be titrated for optimal results in individual systems.
Greenblatt, M.S., et al. 1994. Cancer Res.54, 4855.
Lane, D.P. 1992. Nature358, 15.
Lane, D.P. 1992. Nature358, 15.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
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存储类别
11 - Combustible Solids
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WGK 1
闪点 (F)
Not applicable
闪点 (°C)
Not applicable