CN
生物来源
mouse
质量水平
抗体形式
purified antibody
抗体产品类型
primary antibodies
克隆
F235-1.7.1, monoclonal
形式
liquid
包含
≤0.1% sodium azide as preservative
种属反应性
rat, human, mouse
制造商/商品名称
Calbiochem®
储存条件
do not freeze
同位素/亚型
IgG1
运输
wet ice
储存温度
2-8°C
靶向翻译后修饰
unmodified
基因信息
human ... HRAS(3265)
一般描述
Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with P3X63 Ag8.653 mouse myeloma cells. Recognizes the ~21 kDa c-H-Ras protein.
Recognizes the ~21 kDa c-H-Ras protein in normal skin tissue and Rat1 cells. Does not react with c-K-Ras or c-N-Ras p21. A doublet may be seen due to farnesylation.
This Anti-c-H-Ras (Ab-1) Mouse mAb (F235-1.7.1) is validated for use in Frozen Sections, Immunoblotting, IF, IP, Paraffin Sections for the detection of c-H-Ras (Ab-1).
免疫原
Epitope: within amino acids 54-188
recombinant p21 Ras
应用
Frozen Sections (5 µg/ml)
Immunoblotting (10 µg/ml)
Immunofluorescence (2.5 µg/ml)
Immunoprecipitation (5 µg/ml)
Paraffin Sections (5 µg/ml, saponin and pepsin pre-treatment required)
Immunoblotting (10 µg/ml)
Immunofluorescence (2.5 µg/ml)
Immunoprecipitation (5 µg/ml)
Paraffin Sections (5 µg/ml, saponin and pepsin pre-treatment required)
包装
Please refer to vial label for lot-specific concentration.
警告
Toxicity: Standard Handling (A)
外形
In 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.4.
分析说明
Positive Control
Normal skin tissue or ras 1 cells
Normal skin tissue or ras 1 cells
其他说明
For immunoprecipitation, use 5 µg of Cat. No. OP23 per sample with 45 µl protein G plus agarose. The level of expression of p21Ras is variable in different tissues. For this reason, we recommend a concentration step prior to immunoblotting analysis to obtain optimal results. A doublet may be seen due to farnysylation. Recognizes c-H-Ras and, weakly, v-H-Ras; does not recognize either c-K-Ras or c-N-Ras p21s under the conditions tested. Antibody should be titrated for optimal results in individual systems.
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Kraus, M.H., et al. 1984. Proc. Natl. Acad. Sci. USA81, 5384.
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Bos, J.L. 1989. Cancer Res.49, 4682.
Furth, M. E., et al. 1987. Oncogene1, 47.
Kraus, M.H., et al. 1984. Proc. Natl. Acad. Sci. USA81, 5384.
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法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
储存分类代码
12 - Non Combustible Liquids
WGK
nwg
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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货号
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25G
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