Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with P3X63 Ag8.653 mouse myeloma cells. Recognizes the ~21 kDa c-H-Ras protein.

Recognizes the ~21 kDa c-H-Ras protein in normal skin tissue and Rat1 cells. Does not react with c-K-Ras or c-N-Ras p21. A doublet may be seen due to farnesylation.

This Anti-c-H-Ras (Ab-1) Mouse mAb (F235-1.7.1) is validated for use in Frozen Sections, Immunoblotting, IF, IP, Paraffin Sections for the detection of c-H-Ras (Ab-1).

Epitope: within amino acids 54-188

recombinant p21 Ras

Frozen Sections (5 µg/ml)

Immunoblotting (10 µg/ml)

Immunofluorescence (2.5 µg/ml)

Immunoprecipitation (5 µg/ml)

Paraffin Sections (5 µg/ml, saponin and pepsin pre-treatment required)

Please refer to vial label for lot-specific concentration.

In 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.4.

Positive Control
Normal skin tissue or ras 1 cells

For immunoprecipitation, use 5 µg of Cat. No. OP23 per sample with 45 µl protein G plus agarose. The level of expression of p21Ras is variable in different tissues. For this reason, we recommend a concentration step prior to immunoblotting analysis to obtain optimal results. A doublet may be seen due to farnysylation. Recognizes c-H-Ras and, weakly, v-H-Ras; does not recognize either c-K-Ras or c-N-Ras p21s under the conditions tested. Antibody should be titrated for optimal results in individual systems.

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CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Toxicity: Standard Handling (A)