OP23
Anti-c-H-Ras (Ab-1) Mouse mAb (F235-1.7.1)
liquid, clone F235-1.7.1, Calbiochem®
登录 查看组织和合同定价。
选择尺寸
关于此项目
NACRES:
NA.41
UNSPSC Code:
12352203
Clone:
F235-1.7.1, monoclonal
Species reactivity:
rat, human, mouse
Application:
—
Citations:
21
biological source
mouse
antibody form
purified antibody
antibody product type
primary antibodies
clone
F235-1.7.1, monoclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity
rat, human, mouse
manufacturer/tradename
Calbiochem®
storage condition
do not freeze
dilution
(Frozen Sections (5 µg/mL)
Immunoblotting (10 µg/mL)
Immunofluorescence (2.5 µg/mL)
Immunoprecipitation (5 µg/mL)
Paraffin Sections (5 µg/mL, saponin and pepsin pre-treatment required))
isotype
IgG1
shipped in
wet ice
Quality Level
Gene Information
human ... HRAS(3265)
target post-translational modification
unmodified
General description
Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with P3X63 Ag8.653 mouse myeloma cells. Recognizes the ~21 kDa c-H-Ras protein.
Recognizes the ~21 kDa c-H-Ras protein in normal skin tissue and Rat1 cells. Does not react with c-K-Ras or c-N-Ras p21. A doublet may be seen due to farnesylation.
This Anti-c-H-Ras (Ab-1) Mouse mAb (F235-1.7.1) is validated for use in Frozen Sections, Immunoblotting, IF, IP, Paraffin Sections for the detection of c-H-Ras (Ab-1).
Immunogen
Epitope: within amino acids 54-188
recombinant p21 Ras
Application
Frozen Sections (5 µg/ml)
Immunoblotting (10 µg/ml)
Immunofluorescence (2.5 µg/ml)
Immunoprecipitation (5 µg/ml)
Paraffin Sections (5 µg/ml, saponin and pepsin pre-treatment required)
Immunoblotting (10 µg/ml)
Immunofluorescence (2.5 µg/ml)
Immunoprecipitation (5 µg/ml)
Paraffin Sections (5 µg/ml, saponin and pepsin pre-treatment required)
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.4.
Analysis Note
Positive Control
Normal skin tissue or ras 1 cells
Normal skin tissue or ras 1 cells
Other Notes
For immunoprecipitation, use 5 µg of Cat. No. OP23 per sample with 45 µl protein G plus agarose. The level of expression of p21Ras is variable in different tissues. For this reason, we recommend a concentration step prior to immunoblotting analysis to obtain optimal results. A doublet may be seen due to farnysylation. Recognizes c-H-Ras and, weakly, v-H-Ras; does not recognize either c-K-Ras or c-N-Ras p21s under the conditions tested. Antibody should be titrated for optimal results in individual systems.
Rodenhuis, S. et al. 1992. Cancer Res. (Suppl) 52, 2665s.
Sidransky, D., et al. 1992. Science256, 102.
Bos, J.L. 1989. Cancer Res.49, 4682.
Furth, M. E., et al. 1987. Oncogene1, 47.
Kraus, M.H., et al. 1984. Proc. Natl. Acad. Sci. USA81, 5384.
Shimizu, K., et al. 1983. Proc. Natl. Acad. Sci. USA80, 2112.
Taparowsky, E., et al. 1983. Cell34, 581.
Ellis, R.W., et al. 1981. Nature292, 506.
Shih, T.Y., et al. 1980. Nature287, 686.
Sidransky, D., et al. 1992. Science256, 102.
Bos, J.L. 1989. Cancer Res.49, 4682.
Furth, M. E., et al. 1987. Oncogene1, 47.
Kraus, M.H., et al. 1984. Proc. Natl. Acad. Sci. USA81, 5384.
Shimizu, K., et al. 1983. Proc. Natl. Acad. Sci. USA80, 2112.
Taparowsky, E., et al. 1983. Cell34, 581.
Ellis, R.W., et al. 1981. Nature292, 506.
Shih, T.Y., et al. 1980. Nature287, 686.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
未找到合适的产品？
试试我们的产品选型工具.
存储类别
10 - Combustible liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
Elisa de Stanchina et al.
Molecular cell, 13(4), 523-535 (2004-03-03)
The p53 tumor suppressor promotes cell cycle arrest or apoptosis in response to stress. Previous work suggests that the promyelocytic leukemia gene (PML) can act upstream of p53 to enhance transcription of p53 targets by recruiting p53 to nuclear bodies
Soon Young Shin et al.
Carcinogenesis, 33(12), 2467-2476 (2012-10-03)
Interleukin-11 (IL-11), which belongs to a class of IL6-type cytokines, plays an important role in inflammation, motility and invasion in cancer. The ras mutation is frequently found in human cancer, but little is known regarding the transcriptional activation of the
Yojiro Kotake et al.
Cytotechnology, 68(4), 1591-1596 (2014-12-17)
Recent ultrahigh-density tiling array and large-scale transcriptome analysis have revealed that large numbers of long non-coding RNAs (lncRNAs) are transcribed in mammals. Several lncRNAs have been implicated in transcriptional regulation, organization of nuclear structure, and post-transcriptional processing. However, the regulation
Masahiro Shimizu et al.
Scientific reports, 12(1), 2910-2910 (2022-02-23)
Cancer stem cells (CSCs) have tumour initiation, self-renewal, and long-term tumour repopulation properties, and it is postulated that differentiated somatic cells can be reprogrammed to CSCs by oncogenic signals. We previously showed that oncogenic HRASV12 conferred tumour initiation capacity in
Bohuan Zhong et al.
Environmental toxicology, 38(2), 381-391 (2022-12-01)
Hydroquinone (HQ) is one of the major metabolites of benzene and can cause abnormal gene expression. It is a known carcinogen that alters cell cycle disruption and cell proliferation. However, its chemical mechanism remain a mystery. Circular RNAs (circRNAs) are
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系客户支持