biological source
mouse
clone
CC-1, monoclonal
form
liquid
contains
≤0.1% sodium azide
species reactivity
human, mouse, rat
manufacturer/tradename
Calbiochem®
storage condition
do not freeze
technique(s)
immunocytochemistry: suitable
immunofluorescence: suitable
immunohistochemistry (frozen sections): suitable
dilution
(Free-floating SectionsParaffin Sections)
isotype
IgG2b
shipped in
wet ice
storage temp.
2-8°C
Quality Level
Analysis Note
Negative control: MOPC 21 cells
Positive control: Purkinje cells or cerebellum tissue
Positive control: Purkinje cells or cerebellum tissue
Application
Frozen Sections (5 µg/ml) Immunofluorescence (5 µg/ml) Paraffin Sections (5 µg/ml, heat pre-treament required) Immunocytochemistry (see application references) Free Floating Sections (see application references)
Well suited for IHC and IF studies of oligodendrocytes and optic nerves due to the antibody′s staining of the cell body as opposed to the myelinated processes. Both frozen sections and formalin-fixed, paraffin-embedded brain sections can be used. Antibody should be titrated for optimal results in individual systems.
Well suited for IHC and IF studies of oligodendrocytes and optic nerves due to the antibody′s staining of the cell body as opposed to the myelinated processes. Both frozen sections and formalin-fixed, paraffin-embedded brain sections can be used. Antibody should be titrated for optimal results in individual systems.
Disclaimer
Toxicity: Standard Handling
General description
Recognizes APC in oligodendrocytes and astrocytes.
- Antibody Target Gene Symbol: APC
- Target Synonym: AI047805, Apc7, AU020952, AW124434, BTPS2, DP2, DP2.5, DP3, Familial adenomatous polyposis, FAP, GS, Min, RATAPC
- Entrez Gene Name: adenomatous polyposis coli
- Hu Entrez ID: 324 (Related Antibodies: ST1150, OP62, OP47L, OP44)
- Mu Entrez ID: 11789
- RatEntrez ID: 24205
Immunogen
a recombinant protein consisting of amino acids 1-226 of APC
Packaging
Please refer to vial label for lot-specific concentration
Physical form
In 50 mM sodium phosphate buffer, 0.2% gelatin.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
存储类别
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
L K Su et al.
Cancer research, 53(12), 2728-2731 (1993-06-15)
Germline mutations of the APC gene are responsible for familial adenomatous polyposis, an autosomal dominant inherited predisposition to colorectal tumors. Mutation of the APC gene is also an early, if not initiating, event for sporadic colorectal tumorigenesis. In both cases
K J Smith et al.
Cancer research, 54(14), 3672-3675 (1994-07-15)
The adenomatous polyposis coli protein (APC) is mutated in familial adenomatous polyposis patients as well as in sporadic colorectal tumors. In an attempt to further understand the function of APC, the subcellular localization of APC was examined. Wild-type and mutant
High levels of expression of the tumor suppressor gene APC during development of the rat central nervous system
Bhat, R.V., et al.
The Journal of Neuroscience, 14, 3059-3071 (1994)
L K Su et al.
Science (New York, N.Y.), 262(5140), 1734-1737 (1993-12-10)
Mutations of APC appear to initiate sporadic and inherited forms of human colorectal cancer. Although these mutations have been well characterized, little is known about the function of the APC gene product. Two cellular proteins that associate with APC were
J Groden et al.
Cell, 66(3), 589-600 (1991-08-09)
DNA from 61 unrelated patients with adenomatous polyposis coli (APC) was examined for mutations in three genes (DP1, SRP19, and DP2.5) located within a 100 kb region deleted in two of the patients. The intron-exon boundary sequences were defined for
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