产品名称
Protein A-Turbo - Proximity Biotinylation Enzyme,
form
liquid
purified by
affinity chromatography
packaging
pkg of 100 μg
technique(s)
affinity binding assay: suitable, immunocytochemistry: suitable, immunoprecipitation (IP): suitable, mass spectrometry (MS): suitable, proximity ligation assay: suitable
storage temp.
-65 to -96°C
Quality Level
General description
Protein A Turbo is a recombinantly expressed Protein A-TurboID fusion protein with the capability to bind IgG through the Protein A region, and enzymatically biotinylate proximal proteins non-specifically with the TurboID region.
Application
- Proximity protein labeling
- Cell Biology
- Histology
- Fixed cell microscopy
- Tissue staining
- Immunofluorescence
- Liquid Chromatography
- Mass Spectrometry
- Immunoprecipitation
Biochem/physiol Actions
Protein A strongly binds multiple Immunoglobulin G subclasses (IgG1, IgG2, IgG4), but does not bind IgG3. This probe is not compatible with primary antibodies that are IgG3.
Physical form
Purified protein in 20 mM HEPES pH 7.5, 150 mM NaCl, 0.5 mM Spermidine, 150 mM imidazole with protease inhibitor cocktail.
Preparation Note
0.7 mg/mL
Store at -65°C to -96°C.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2
存储类别
6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Irene Santos-Barriopedro et al.
Nature communications, 12(1), 5015-5015 (2021-08-20)
Proximity biotinylation workflows typically require CRISPR-based genetic manipulation of target cells. To overcome this bottleneck, we fused the TurboID proximity biotinylation enzyme to Protein A. Upon target cell permeabilization, the ProtA-Turbo enzyme can be targeted to proteins or post-translational modifications
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