biological source
rabbit
antibody form
purified antibody
antibody product type
primary antibodies
clone
polyclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity
human, mouse, rat
manufacturer/tradename
Calbiochem®
storage condition
do not freeze
isotype
IgG
shipped in
wet ice
Quality Level
Gene Information
human ... FOS(2353)
target post-translational modification
unmodified
General description
原癌基因fos与细胞的生长，分化和发育有关。Fos是由大量刺激物诱导的，包括有丝分裂原，分化特异性药物，药理药物等。62,000道尔顿fos蛋白的诱导是快速但短暂的。fos蛋白与39,000道尔顿蛋白相关，该蛋白已被鉴定为jun原癌基因（c-jun）的蛋白产物。Fos/Jun蛋白复合物与DNA中称为AP-1结合位点的序列元件特异性结合。
可识别HepG2、NIH3T3和MCF-7细胞中的~50-62 kDa c-Fos蛋白。
蛋白A纯化的兔多克隆抗体。可识别v-fos和~50-62 kDa c-fos蛋白。
该抗c-Fos（Ab-2）（4-17）兔pAb经验证可用于冰冻切片、免疫印迹、IF、IP、石蜡切片，以检测c-Fos（Ab-2）（4-17）。
Immunogen
人
对应于人c-Fos氨基酸4-17的合成肽（SGFNADYEASSSRC）（目录号PP10）
Application
冰冻切片（5-10 µg/ml）
免疫印迹（1-5 µg/ml）
免疫荧光（1-5 µg/ml）
免疫沉淀（1-2 µg/反应）
石蜡切片（5-10 µg/ml，需要胃蛋白酶或加热预处理）
自由浮动切片（不建议使用）
免疫印迹（1-5 µg/ml）
免疫荧光（1-5 µg/ml）
免疫沉淀（1-2 µg/反应）
石蜡切片（5-10 µg/ml，需要胃蛋白酶或加热预处理）
自由浮动切片（不建议使用）
Packaging
请参考特定浓度批号的标签。
Physical form
溶于0.05 M磷酸钠缓冲液，含0.2％明胶。
Analysis Note
阳性对照
HepG2、NIH 3T3细胞、MCF-7细胞、结肠或乳腺腺癌组织
HepG2、NIH 3T3细胞、MCF-7细胞、结肠或乳腺腺癌组织
Other Notes
对于凝胶阻滞分析，使用目录编号PC05L重悬于100 µl缓冲液中。免疫原，c-fos（肽-2），目录编号PP10也可用于竞争研究（请参见下面的Murphy等人）。在单个系统中，应对抗体进行滴定以获得最佳结果。
DeTogni, P., et al. 1988.Cell Biol.8, 2251.
Rouscher III, F.J., et al. 1988.Science240, 1010.
Sassone-Corsi, P., et al. 1988.Cell54, 553.
Verma, I.M. and Graham, W.R.1987.Cancer Res.49, 29.
Verma, I.M. and Sassone-Corsi, P. 1987.Cell51, 513.
Muller, R. 1986.Biochim.Biophys.Acta823, 207.
Verma, I. 1986.Trends Genet.2, 93.
Greenberg, M. and Ziff, E. 1984.Nature (London), 311, 433.
Rouscher III, F.J., et al. 1988.Science240, 1010.
Sassone-Corsi, P., et al. 1988.Cell54, 553.
Verma, I.M. and Graham, W.R.1987.Cancer Res.49, 29.
Verma, I.M. and Sassone-Corsi, P. 1987.Cell51, 513.
Muller, R. 1986.Biochim.Biophys.Acta823, 207.
Verma, I. 1986.Trends Genet.2, 93.
Greenberg, M. and Ziff, E. 1984.Nature (London), 311, 433.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
毒性：高毒性（H）
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存储类别
10 - Combustible liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
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