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Merck
CN

PC05

抗-c-Fos (Ab-2) (4-17) 兔pAb

liquid, Calbiochem®

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关于此项目

NACRES:
NA.41
UNSPSC Code:
12352203
Clone:
polyclonal
Species reactivity:
human, mouse, rat
Application:
Citations:
42
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biological source

rabbit

Quality Level

antibody form

purified antibody

antibody product type

primary antibodies

clone

polyclonal

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity

human, mouse, rat

manufacturer/tradename

Calbiochem®

storage condition

do not freeze

isotype

IgG

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

Gene Information

human ... FOS(2353)

General description

原癌基因fos与细胞的生长,分化和发育有关。Fos是由大量刺激物诱导的,包括有丝分裂原,分化特异性药物,药理药物等。62,000道尔顿fos蛋白的诱导是快速但短暂的。fos蛋白与39,000道尔顿蛋白相关,该蛋白已被鉴定为jun原癌基因(c-jun)的蛋白产物。Fos/Jun蛋白复合物与DNA中称为AP-1结合位点的序列元件特异性结合。
可识别HepG2、NIH3T3和MCF-7细胞中的~50-62 kDa c-Fos蛋白。
蛋白A纯化的兔多克隆抗体。可识别v-fos和~50-62 kDa c-fos蛋白。
该抗c-Fos(Ab-2)(4-17)兔pAb经验证可用于冰冻切片、免疫印迹、IF、IP、石蜡切片,以检测c-Fos(Ab-2)(4-17)。

Immunogen

对应于人c-Fos氨基酸4-17的合成肽(SGFNADYEASSSRC)(目录号PP10)

Application

冰冻切片(5-10 µg/ml)

免疫印迹(1-5 µg/ml)

免疫荧光(1-5 µg/ml)

免疫沉淀(1-2 µg/反应)

石蜡切片(5-10 µg/ml,需要胃蛋白酶或加热预处理)

自由浮动切片(不建议使用)

Packaging

请参考特定浓度批号的标签。

Physical form

溶于0.05 M磷酸钠缓冲液,含0.2%明胶。

Analysis Note

阳性对照
HepG2、NIH 3T3细胞、MCF-7细胞、结肠或乳腺腺癌组织

Other Notes

对于凝胶阻滞分析,使用目录编号PC05L重悬于100 µl缓冲液中。免疫原,c-fos(肽-2),目录编号PP10也可用于竞争研究(请参见下面的Murphy等人)。在单个系统中,应对抗体进行滴定以获得最佳结果。
DeTogni, P., et al. 1988.Cell Biol.8, 2251.
Rouscher III, F.J., et al. 1988.Science240, 1010.
Sassone-Corsi, P., et al. 1988.Cell54, 553.
Verma, I.M. and Graham, W.R.1987.Cancer Res.49, 29.
Verma, I.M. and Sassone-Corsi, P. 1987.Cell51, 513.
Muller, R. 1986.Biochim.Biophys.Acta823, 207.
Verma, I. 1986.Trends Genet.2, 93.
Greenberg, M. and Ziff, E. 1984.Nature (London), 311, 433.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

毒性:高毒性(H)


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A R Soderman et al.
Neuroscience, 154(4), 1506-1516 (2008-06-14)
Opioid receptor agonists and antagonists have profound effects on cocaine-induced hyperactivity and conditioned reward. Recently, the role specifically of the mu opioid receptor has been demonstrated based on the finding that i.c.v. administration of the selective mu opioid receptor antagonist
N Laflamme et al.
Journal of molecular neuroscience : MN, 8(3), 165-179 (1997-06-01)
The purpose of this study was to investigate the effect of the immune activator lipopolysaccharide (LPS) on the expression of corticotropin-releasing factor (CRF) and glucocorticoid receptor (GR) mRNA in the paraventricular nucleus (PVN) of transgenic mice with impaired GR function
Natasha M Sosanya et al.
BMC neuroscience, 20(1), 17-17 (2019-04-25)
Reports show that stressful events before injury exacerbates post-injury pain. The mechanism underlying stress-induced heightened thermal pain is unclear. Here, we examined the effects of chronic intermittent stress (CIS) on nociceptive behaviors and brain-derived nerve growth factor (BDNF) system in