Anti-c-Fos (Ab-2) (4-17) Rabbit pAb

Immunoaffinity purified rabbit polyclonal antibody. Recognizes the ~50-62 kDa c-Fos protein.

Recognizes the ~50-62 kDa c-Fos protein in HepG2, NIH3T3, and MCF-7 cells.

The proto-oncogene fos has been implicated in cell growth, differentiation and development. fos is induced by a large number of stimuli including mitogens, differentiation-specific agents, pharmacological agents, etc. Induction of the 62,000 dalton fos protein is rapid but transient. The fos protein is associated with a 39,000 dalton protein which has been identified as the protein product of the jun proto-oncogene (c-jun). The fos/jun protein complex binds specifically to a sequence element in DNA referred to as the AP-1 binding site.

This Anti-c-Fos (Ab-2) (4-17) Rabbit pAb is validated for use in Frozen Sections, WB, IF, IP, Paraffin Sections, Gel Shift, for the detection of c-Fos (Ab-2) (4-17).

Human

a synthetic peptide (SGFNADYEASSSRC) (Cat. No. PP10) corresponding to amino acids 4-17 of human c-Fos

Frozen Sections (5-10 µg/ml)

Immunoblotting (1-5 µg/ml)

Immunofluorescence (1-5 µg/ml)

Immunoprecipitation (1-2 µg per reaction)

Paraffin Sections (5-10 µg/ml, heat or pepsin pre-treatment required)

Gel Shift (see comments and application references)

Free-floating Sections (not recommended)

Toxicity: Standard Handling (A)

Lyophilized from PBS, a volatile buffer, 100 µg BSA.

Resuspend the lyophilized antibody with sterile phosphate buffered saline (PBS), pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml; product will be more stable if 0.1% sodium azide is included (do not add azide if antibody is to be used with viable cells). Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing.

Positive Control
Hep G2 or NIH 3T3 cells

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Rouscher III, F.J., et al. 1988. Science240, 1010.
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Verma, I.M. and Graham, W.R. 1987. Cancer Res.49, 29.
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Muller, R. 1986. Biochim. Biophys. Acta823, 207.
Verma, I. 1986. Trends Genet.2, 93.
Greenberg, M. and Ziff, E. 1984. Nature (London), 311, 433

For gel shift assays resuspend in 100 µl of buffer. The immunogen, c-fos (Peptide-2), Cat. No. PP10, is also available for competition studies (see Murphy, et al. below). Antibodies should be titrated for optimal results in individual systems.

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