产品名称
Anti-NF-κB (p65, RelA) (Ab-2) Rabbit pAb, liquid, Calbiochem®
biological source
rabbit
antibody product type
primary antibodies
clone
polyclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity
human
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
avoid repeated freeze/thaw cycles
dilution
(ELISA
Gel Shift
Immunoblotting (1:500, chemiluminescence)
Immunoprecipitation )
isotype
IgG
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Analysis Note
Positive Control
Daudi cells
Daudi cells
Application
ELISA (see comments)
Gel Shift (see comments)
Immunoblotting (1:500, chemiluminescence)
Immunoprecipitation (see comments)
Gel Shift (see comments)
Immunoblotting (1:500, chemiluminescence)
Immunoprecipitation (see comments)
Disclaimer
Toxicity: Standard Handling (A)
General description
Rabbit polyclonal antibody. Recognizes the ~65 kDa NF-κB p65 (RelA) protein.
Recognizes the ~65 kDa NF-κB p65 (RelA) protein in Daudi cells.
This Anti-NF-κB (p65, RelA) (Ab-2) Rabbit pAb is validated for use in ELISA, Gel Shift, Immunoblotting, Immunoprecipitation for the detection of NF-κB (p65, RelA) (Ab-2).
Immunogen
Human
a synthetic peptide corresponding to amino acids near the N-terminal domain of human NF-κB p65 (RelA), conjugated to KLH
Other Notes
Kolberg, R., 1997. J. NIH Research9, 29.
Van Antwerp, D.J. et al. 1996. Science274, 787.
Beg, A.A., et al. 1995. Nature376, 167.
Baeuerle, P.A., and Henkel, T., 1994. Ann. Rev. Immunol.12, 141.
Wulczyn, F.G., et al. 1992. Nature358, 597.
Van Antwerp, D.J. et al. 1996. Science274, 787.
Beg, A.A., et al. 1995. Nature376, 167.
Baeuerle, P.A., and Henkel, T., 1994. Ann. Rev. Immunol.12, 141.
Wulczyn, F.G., et al. 1992. Nature358, 597.
This antibody has also been reported to work for ELISA, gel shift, and immunoprecipitation. Antibody should be titrated for optimal results in individual systems.
Physical form
In 5 mM sodium phosphate buffer, pH 7.4.
Preparation Note
Following initial thaw, aliquot and freeze (-20°C).
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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