biological source
rabbit
Quality Level
antibody form
serum
antibody product type
primary antibodies
clone
polyclonal
form
lyophilized
does not contain
preservative
species reactivity
rat
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
dilution
(Frozen Sections (1:500-1:1000, Cy3 technique; 1:3000-1:6000, HRP)
Immunoblotting (1:2000-1:2500;
Immunofluorescence (1:100-1:200)
Immunoprecipitation )
isotype
IgG
shipped in
ambient
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
rat ... Oprm1(25601)
General description
Rabbit polyclonal antibody supplied as lyophilized, undiluted serum. Recognizes the ~44-45 kDa opioid µ receptor protein.
Recognizes the ~44-45 kDa opiod μ receptor in caudate putamen and the dorsal horn of the spinal cord.
This Anti-Opioid µ Receptor (384-398) Rabbit pAb is validated for use in Frozen Sections, Immunoblotting, IF, IP for the detection of Opioid µ Receptor (384-398).
Immunogen
Rat
a synthetic peptide corresponding to amino acids 384-398 of rat opioid µ receptor
Application
Frozen Sections (1:500-1:1000, Cy3 technique; 1:3000-1:6000, HRP)
Immunoblotting (1:2000-1:2500; see application references)
Immunofluorescence (1:100-1:200)
Immunoprecipitation (see comments)
Immunoblotting (1:2000-1:2500; see application references)
Immunofluorescence (1:100-1:200)
Immunoprecipitation (see comments)
Physical form
Undiluted serum.
Preparation Note
Reconstitute the lyophilized antibody with 100 µl sterile distilled H₂O. Resulting reconstituted solution contains ≤0.1% sodium azide. Be careful to reconstitute the entire contents of the vial; during shipment and handling portions of the lyophilized pellet may have become dislodged and may not be in the bottom of the vial. Following reconstitution, aliquot and freeze (-20°C).
Analysis Note
Positive Control
Rat caudate putamen or spinal cord (dorsal horn)
Rat caudate putamen or spinal cord (dorsal horn)
Other Notes
The specificity was determined by immunolabeling of transfected cells, immunoblotting analysis, and immunoisolation studies. Antibody specificity was examined in the rat caudate putamen and dorsal horn of the spinal cord. Staining is completely eliminated by pre-treatment of antibody with immunogen peptide at a concentration of 10 µg/ml. This antibody has also been reported to work for immunopercipitation. Antibody should be titrated for optimal results in individual systems.
Zaki, P.A., et al. 1996. Annu. Rev. Pharmacol. Toxicol.36, 379.
Arvidsson, U., et al. 1995. J. Neurosci.15, 3328.
Kieffer, B.L. 1995. Cell. Mol. Neurobiol.15, 615-635.
Childers, S.R. 1991. Life Sci.48, 1991.
Arvidsson, U., et al. 1995. J. Neurosci.15, 3328.
Kieffer, B.L. 1995. Cell. Mol. Neurobiol.15, 615-635.
Childers, S.R. 1991. Life Sci.48, 1991.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Highly Toxic (H)
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存储类别
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Heng Xu et al.
The Journal of pharmacology and experimental therapeutics, 315(1), 248-255 (2005-07-01)
A growing body of literature indicates that chronic morphine exposure alters the expression and function of cytoskeletal proteins in addition to the well established interactions between mu opioid receptors and G proteins. In the present study, we hypothesized that chronic
Belén Gago et al.
Journal of neuroscience research, 91(12), 1533-1540 (2013-09-17)
The peptides dynorphin and enkephalin modulate many physiological processes, such as motor activity and the control of mood and motivation. Their expression in the caudate putamen (CPu) is regulated by dopamine and opioid receptors. The current work was designed to
Heng Xu et al.
Synapse (New York, N.Y.), 61(3), 166-175 (2006-12-08)
Previous studies established that Tyr-D-Ala-Gly-N-Me-Phe-Gly-ol (DAMGO) and (2S,4aR,6aR,7R,9S,10aS,10bR)-9-(Benzoyloxy)-2-(3-furanyl)dodecahydro-6a,10b-dimethyl-4,10-dioxo-2H-naphtho-[2,1-c]pyran-7-carboxylic acid methyl ester (herkinorin) are fully efficacious mu-agonists. Herkinorin (HERK), unlike DAMGO, does not recruit beta-arrestin and promote mu-receptor internalization, even in cells that over express beta-arrestin. We hypothesized that chronic HERK
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| PC165L-100UL | 04055977209501 |