biological source
rabbit
antibody form
serum
antibody product type
primary antibodies
clone
polyclonal
form
lyophilized
contains
≤0.1% sodium azide as preservative
species reactivity
rat
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
dilution
(Frozen Sections (1:400-1:800, Cy3; 1:2000-1:4000, Bn-SA/HRP)
Immunofluorescence (1:200-1:400))
isotype
IgG
shipped in
ambient
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
rat ... Avp(24221)
General description
Rabbit polyclonal antibody supplied as lyophilized undiluted serum. Recognizes vasopressin.
Recognizes vasopressin in rat hypothalamus.
This Anti-Vasopressin Rabbit pAb is validated for use in Frozen Sections, Immunofluorescence for the detection of Vasopressin.
Immunogen
Rat
rat arginine vasopressin
Application
Frozen Sections (1:400-1:800, Cy3; 1:2000-1:4000, Bn-SA/HRP)
Immunofluorescence (1:200-1:400)
Physical form
Undiluted serum.
Preparation Note
Reconstitute the lyophilized antibody with 100 µl sterile distilled H₂O. Be careful to reconstitute the entire contents of the vial; during shipment and handling portions of the lyophilized pellet may have become dislodged and may not be in the bottom of the vial. Dilute with sterile PBS or Tris buffer at dilutions no higher than 1:10. Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months. Avoid freeze/thaw cycles of solutions.
Analysis Note
Positive Control
Rat hypothalamus
Rat hypothalamus
Other Notes
Antibody specificity was examined in the rat paraventricular nucleus and supraoptic nucleus. Staining is completely eliminated by pretreatment of antibody with arginine vasopressin (10 µg/ml). Preabsorption of antibody with oxytocin (100 µg/ml) resulted in no loss of immunostaining. Antibody should be titrated for optimal results in individual systems.
Sudhof, T.C. 1995. Nature375, 645.
Hokfelt, T., et al. 1987. Experientia Suppl.56, 154.
Hokfelt, T., et al. 1987. Experientia Suppl.56, 154.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
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存储类别
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Yarely C Hoffiz et al.
Scientific reports, 11(1), 2335-2335 (2021-01-29)
Long-standing clinical findings report a dramatic surge of vasopressin in umbilical cord blood of the human neonate, but the neural underpinnings and function(s) of this phenomenon remain obscure. We studied neural activation in perinatal mice and rats, and found that
V Tobin et al.
Journal of neuroendocrinology, 24(4), 629-641 (2011-10-13)
In magnocellular neurones of the supraoptic nucleus (SON), the neuropeptides vasopressin and oxytocin are synthesised and packaged into large dense-cored vesicles (LDCVs). These vesicles undergo regulated exocytosis from nerve terminals in the posterior pituitary gland and from somata/dendrites in the
Zita Liutkevičiūtė et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, fj201800443-fj201800443 (2018-06-26)
Ants are emerging model systems to study cellular signaling because distinct castes possess different physiologic phenotypes within the same colony. Here we studied the functionality of inotocin signaling, an insect ortholog of mammalian oxytocin (OT), which was recently discovered in
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Molecular neurodegeneration, 14(1), 8-8 (2019-02-10)
Neurotropic virus-based tracers have been extensively applied in mapping and manipulation of neural circuits. However, their neurotropic and neurotoxic properties remain to be fully characterized. Through neural circuit tracing, we systematically compared the neurotropism discrepancy among different multi-trans-synaptic and mono-synaptic
Alfonso Reimúndez et al.
Acta physiologica (Oxford, England), 237(3), e13896-e13896 (2022-10-18)
Physiological functions in mammals show circadian oscillations, synchronized by daily cycles of light and temperature. Central and peripheral clocks participate in this regulation. Since the ion channel TRPM8 is a critical cold sensor, we investigated its role in circadian function.
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