生物来源
rabbit
质量水平
抗体形式
affinity isolated antibody
抗体产品类型
primary antibodies
克隆
polyclonal
表单
liquid
包含
≤0.1% sodium azide as preservative
种属反应性
(GFP and some GFP fusions)
制造商/商品名称
Calbiochem®
储存条件
OK to freeze
avoid repeated freeze/thaw cycles
同位素/亚型
IgG
运输
wet ice
储存温度
−20°C
靶向翻译后修饰
unmodified
一般描述
Anti-Green Fluorescent Protein (26-39), rabbit polyclonal, recognizes the ~28 kDa GFP and some GFP fusion proteins. It is validated for use in Western blotting and immunocytochemistry.
Immunoaffinity purified rabbit polyclonal antibody. Recognizes the ~28 kDa GFP and some GFP fusion proteins.
Recognizes the ~28 kDa GFP and some GFP fusion proteins.
免疫原
Aequora victoria
a synthetic peptide corresponding to amino acids 26-39 of Aequorea victoria wild-type green fluorescent protein
应用
Immunoblotting (1:2000)
Immunocytochemistry (1:200)
Immunocytochemistry (1:200)
包装
Please refer to vial label for lot-specific concentration.
警告
Toxicity: Standard Handling (A)
外形
In PBS, 1 mg/ml BSA.
重悬
Following initial thaw, aliquot and freeze (-20°C).
其他说明
Cubitt, A.B., et al. 1995. Trends Biochem. Sci.20, 448.
This sequence is completely conserved between GFP and the modified and enhanced Blue, Green, and Yellow FP variants. This antibody has been successfully used in immunoblotting and immunocytochemistry protocols to detect GFP and fusion proteins. It detects a ~28 kDa protein that corresponds to GFP in immunoblots of protein extract from cells transfected with, and overexpressing, GFP isoforms. Antibody should be titrated for optimal results in individual sytems.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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储存分类代码
10 - Combustible liquids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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