biological source
rabbit
Quality Segment
antibody form
serum
antibody product type
primary antibodies
clone
polyclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity
human
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, avoid repeated freeze/thaw cycles
dilution
(Immunoprecipitation (1:250-1:1000))
isotype
IgG
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... PCNA(5111)
General description
Rabbit polyclonal antibody supplied as undiluted serum. Recognizes the ~36-37 kDa PCNA protein.
Recognizes the ~37 kDa PCNA protein in MCF-7 cells.
This Anti-PCNA (247-261) (Ab-5) Rabbit pAb is validated for use in Immunoprecipitation for the detection of PCNA (247-261) (Ab-5).
Immunogen
Human
a synthetic peptide corresponding to amino acids 247-261 of human PCNA
Application

Immunoprecipitation (1:250-1:1000)
Physical form
Undiluted serum.
Preparation Note
Following initial thaw, aliquot and freeze (-20°C).
Analysis Note
Positive Control
MCF7 cells
MCF7 cells
Other Notes
Miyachi, K., et al. 1990. J. Immunol.121, 2228.
Waseem, N.H. and Lane, D.P. 1990. Science96, 121.
Suzuka, I., et al. 1989. Proc. Natl. Acad. Sci. USA86, 3189.
Bravo, R., et al. 1987. Nature (London)326, 515.
Bravo, R. and MacDonald-Bravo, H. 1987. J. Cell. Biol.105, 1549.
Waseem, N.H. and Lane, D.P. 1990. Science96, 121.
Suzuka, I., et al. 1989. Proc. Natl. Acad. Sci. USA86, 3189.
Bravo, R., et al. 1987. Nature (London)326, 515.
Bravo, R. and MacDonald-Bravo, H. 1987. J. Cell. Biol.105, 1549.
Will immunoprecipitate PCNA when complexed with other proteins. Antibody should be titrated for optimal results in individual systems.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
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存储类别
10 - Combustible liquids
wgk
WGK 1