PC635
PhosphoDetect Anti-NBS1 (pSer³⁴³) Rabbit pAb
liquid, Calbiochem®
别名:
Anti-Nibrin (pSer³⁴³) Rabbit pAb
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关于此项目
NACRES:
NA.43
UNSPSC Code:
12352203
Clone:
polyclonal
Species reactivity:
human
Application:
—
Citations:
-
biological source
rabbit
antibody form
diluted serum
antibody product type
primary antibodies
clone
polyclonal
form
liquid
does not contain
preservative
species reactivity
human
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, avoid repeated freeze/thaw cycles
dilution
(ELISA (1:16,000)
Immunoblotting (1:1000)
Immunoprecipitation (1 µL/500 µg cell extract))
isotype
IgG
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
phosphorylation (pSer343)
Quality Level
Gene Information
human ... NBN(4683)
General description
Rabbit polyclonal antibody supplied as adsorbed diluted serum. Recognizes the ~95 kDa nibrin (NBS1) protein phosphorylated at Ser343.
Recognizes the ~95 kDa nibrin protein phosphorylated at Ser343 in δ-irradiated lymphoblastoid cells.
This PhosphoDetect Anti-NBS1 (pSer³⁴³) Rabbit pAb is validated for use in ELISA, Immunoblotting, Immunoprecipitation for the detection of NBS1 (pSer³⁴³).
Immunogen
Human
a synthetic phosphopeptide [TPGPSLpSQGVSVD(C)] corresponding to amino acids 337-349 surrounding the Ser³⁴³ phosphorylation site of human nibrin, conjugated to diphtheria toxoid
Application
ELISA (1:16,000)
Immunoblotting (1:1000)
Immunoprecipitation (1 µl/500 µg cell extract)
Immunoblotting (1:1000)
Immunoprecipitation (1 µl/500 µg cell extract)
Physical form
Serum diluted in PBS.
Preparation Note
Following initial thaw, aliquot and freeze (-20°C).
Analysis Note
Positive Control
γ-irradiated lymphoblastoid cells
γ-irradiated lymphoblastoid cells
Other Notes
Gatei, M., et al. 2000. Nat. Genet.25, 115.
Lim, D.S., et al. 2000. Nature404, 613.
Wu, G., et al. 2000. J. Biol. Chem.275, 30618.
Zhong, Q., et al. 1999. Science285, 747.
Carney, J.P., et al. 1998. Cell93, 477.
Trujillo, K.M., et al. 1998. J. Biol. Chem.273, 21447.
Varon, R., et al. 1998. Cell93, 467.
Lim, D.S., et al. 2000. Nature404, 613.
Wu, G., et al. 2000. J. Biol. Chem.275, 30618.
Zhong, Q., et al. 1999. Science285, 747.
Carney, J.P., et al. 1998. Cell93, 477.
Trujillo, K.M., et al. 1998. J. Biol. Chem.273, 21447.
Varon, R., et al. 1998. Cell93, 467.
This antibody preparation has been adsorbed against non-phosphopeptide that corresponds to the immunogen sequence. Does not detect non-phosphorylated nibrin protein. Antibody should be titrated for optimal results in individual systems.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
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存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
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