biological source
rabbit
antibody form
serum
antibody product type
primary antibodies
clone
polyclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity
rat, human, hamster, mouse
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, avoid repeated freeze/thaw cycles
dilution
(ELISA (direct only) (1:2000-1:4000)
Immunoblotting (1:500-1:4000)
Immunocytochemistry (1:200-1:1000)
Immunoprecipitation (not recommended))
isotype
IgG
shipped in
wet ice
storage temp.
−70°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... IDE(3416)
General description
Rabbit polyclonal antibody supplied as undiluted serum. Recognizes the ~115 kDa endogenous as well as recombinant IDE protein.
Recognizes the ~115 kDa endogenous and recombinant IDE.
This Anti-IDE, N-Terminal (97-273) Rabbit pAb is validated for use in ELISA, Immunoblotting, Immunocytochemistry, Immunoprecipitation for the detection of IDE, N-Terminal (97-273).
Immunogen
Rat
a recombinant protein consisting of amino acids 97-273 of rat IDE fused to GST
Application
ELISA (direct only) (1:2000-1:4000)
Immunoblotting (1:500-1:4000)
Immunocytochemistry (1:200-1:1000)
Immunoprecipitation (not recommended)
Immunoblotting (1:500-1:4000)
Immunocytochemistry (1:200-1:1000)
Immunoprecipitation (not recommended)
Physical form
Undiluted serum.
Preparation Note
Following initial thaw, aliquot and freeze (-70°C).
Analysis Note
Positive Control
Rat or mouse liver cytosol
Rat or mouse liver cytosol
Other Notes
Antibody should be titrated for optimal results in individual systems.
Morelli, L., et al. 2003. J. Biol. Chem.278, 23221.
Kurochkin, I.V. and Goto, S. 1994. FEBS Lett.345, 33.
Kurochkin, I.V. and Goto, S. 1994. FEBS Lett.345, 33.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
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存储类别
10 - Combustible liquids
wgk
WGK 1
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