PCYTMG-23K-13PX
MILLIPLEX® Porcine Cytokine/Chemokine Magnetic Bead Panel - Immunology Multiplex Assay
Simultaneously analyze multiple cytokine and chemokine biomarkers with Bead-Based Multiplex Assays using the Luminex technology, in porcine serum, plasma and cell culture samples.
别名:
luminex porcine cytokine chemokine growth factor multiplex assay, luminex porcine pro inflammatory anti inflammatory cytokine panel, millipore porcine inflammation multiplex kit, porcine interleukin immunoassay panel
质量水平
种属反应性
pig
制造商/商品名称
Milliplex®
assay range
accuracy: 92-103%
standard curve range: 0.005-20 ng/mL
(IL-1α)
standard curve range: 0.012-50 ng/mL
(IL-8)
standard curve range: 0.024-100 ng/mL
(GM-CSF, IL-1ra, IL-2, IL-6, IL-10, IL-12, IL-18 & TNFα)
standard curve range: 0.061-250 ng/mL
(IL-4)
standard curve range: 0.122-500 ng/mL
(IFNγ & IL-1β)
技术
multiplexing: suitable
相容性
configured for Premixed
检测方法
fluorometric (Luminex xMAP)
运输
wet ice
一般描述
With a similarity to humans in heart size, blood flow rate, skin, liver enzymes and bone healing, porcine models have replaced many canine and non-human primate models in many research areas such as cardiovascular, toxicology, pharmacology, aging and bone. “Minipigs,” bred to retain the similar heart size and function as humans, but age without growing too large, have been used for long term preclinical or multi-generational studies which may be performed in a comparatively short period of time.
The MILLIPLEX® Porcine Cytokine/Chemokine Panel can be used for the simultaneous quantification of all the following analytes in porcine tissue/cell lysate and culture supernatant samples and serum or plasma samples: GM-CSF, IFNγ, IL-1α, IL-1ra, IL-1β, IL-2, IL-4, IL-6, IL-8, IL-10, IL-12, IL-18 and TNFα. This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate.
The Luminex® xMAP® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.
Panel Type: Cytokines/Chemokines
The MILLIPLEX® Porcine Cytokine/Chemokine Panel can be used for the simultaneous quantification of all the following analytes in porcine tissue/cell lysate and culture supernatant samples and serum or plasma samples: GM-CSF, IFNγ, IL-1α, IL-1ra, IL-1β, IL-2, IL-4, IL-6, IL-8, IL-10, IL-12, IL-18 and TNFα. This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate.
The Luminex® xMAP® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.
Panel Type: Cytokines/Chemokines
应用
- Analytes: GM-CSF, IFNγ, IL-1α, IL-1β, IL-1Ra, IL-2, IL-4, IL-6, IL-8 (CXCL8), IL-10, IL-12, IL-18, TNF-α
- Recommended Sample Type: serum, plasma or cell/tissue culture supernatants or lysate
- Recommended Sample Dilution: 25 μL per well of undiluted serum or plasma; cell/tissue culture samples may require dilution in an appropriate control medium.
- NOTE: Extra centrifugation and attention may be needed for samples with extremely high lipid content.
- Assay Run Time: Overnight (16-18 hours) at 2-8°C.
- Research Category: Inflammation & Immunology
生化/生理作用
Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
包装
Premixed beads for your convenience.
制备说明
Recommended storage for kit components is 2 - 8°C.
其他说明
Sensitivity: Refer to kit protocol for sensitivities of individual biomarkers.
法律信息
Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
警示用语：
Danger
危险分类
Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1
储存分类代码
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
法规信息
新产品
此项目有
Cristina A Martinez et al.
Biology, 10(2) (2021-03-07)
Seminal plasma (SP) in the female genital tract induces changes that affect multiple reproductive processes. One of the active components in SP is the transforming growth factor β1 (TGF-β1), which has major roles in embryo development and pregnancy. Embryo transfer
Iliya Shulman et al.
Biology, 10(2) (2021-01-28)
Peripheral blood presents an available source of cells for both fundamental research and clinical use. In our study, we have evaluated the therapeutic potential of peripheral blood mononuclear cells (PBMCs) excluding the preliminary sorting or mobilization of peripheral blood stem
Song-Song Xu et al.
Frontiers in nutrition, 9, 807118-807118 (2022-03-15)
The diet-induced gut microbiota dysbiosis has been suggested as a major risk factor for atherothrombosis, however, the detailed mechanism linking these conditions is yet to be fully understood. We established a long-term excessive-energy diet-induced metabolic syndrome (MetS) inbred Wuzhishan minipig
Yana Mukhamedshina et al.
Biomolecules, 9(12) (2019-12-07)
Here, we provide a first comparative study of the therapeutic potential of allogeneic mesenchymal stem cells derived from bone marrow (BM-MSCs), adipose tissue (AD-MSCs), and dental pulp (DP-MSCs) embedded in fibrin matrix, in small (rat) and large (pig) spinal cord
Measurable Cytokine Concentrations in Pig Seminal Plasma Are Modified by Semen Handling and Storage.
Lorena Padilla et al.
Biology, 9(9) (2020-09-11)
Sample handling and storing are critical steps for the reliable measurement of circulating biomolecules in biological fluids. This study evaluates how cytokine measurements in pig seminal plasma (SP) vary depending on semen handling and SP storage. Thirteen cytokines (GM-CSF, IFNγ
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