物料
angled neck high-density polyethylene cap (vented)
polystyrene flask
质量水平
无菌性
sterile; γ-irradiated
特点
3 layer
包装
pack of 16
制造商/商品名称
Millicell®
参数
45 °C max. temp.
技术
cell culture | mammalian: suitable
高度
32 mm , excluding cap
51 mm , including cap
长度
22.2 cm
宽度
12 cm
表面积
600 cm2
工作体积
120-180 mL
吸附类型
Tissue Culture (TC)-treated surface
运输
ambient
制备说明
- To seed cells, fill the Millicell HY T-600 Flask with the desired growth media.
- Add desired number of cells to achieve typical seeding density.
- Mix the cells and media by raising the end of the flask and pipetting the solution up and down several times until a homogeneous suspension is achieved. Note: Failure to adequately mix cells into a homogeneous suspension may result in an uneven distribution of cells across the layers of the Millicell HY Flask.
- Briefly lay the flask on its side to equilibrate the liquid volume equally among the layers of the flask.
- Tilt the flask on its end to partition the liquid volume for each layer of the flask.
- Lay the flask down to spread the cell suspension evenly across the entire surface of each layer.
- Depending on the volume it may be necessary to rock the flask on all four corners to ensure that the entire surface wets. Note: If the liquid volume on any of the layers changes during the wetting procedure (e.g., spilling of liquid from top layer to lower layers), repeat steps 4–6 to ensure even distribution of liquid among all layers.
- Return the flask to a vertical position when transporting to the incubator. Lay the flask flat in the incubator (as in step 6) and culture cells under appropriate conditions.
- If media exchange is required, either aspirate or pour the media from the Millicell HY T-600 Flask. Note: Due to the larger surface area of the Millicell Flasks, it may be necessary to let the flask stand on its side for several minutes following each aspiration so that all residual liquid pools to a common point, allowing for a second aspiration to completely remove the remaining liquid.
- Add appropriate volume of fresh media, and repeat steps 4–8.
- To harvest cells, remove media as in step 9.
- Add desired amount of appropriate wash solution (e.g., PBS or 0.02% EDTA) to the flask and repeat steps 4–7.
- Remove wash solution as in step 9.
- Add desired amount of dissociation enzyme (e.g., 0.25% trypsin/EDTA or equivalent) according to preferred protocol, repeat steps 4–8, and incubate. Volume of dissociation enzyme required per cm2 does not need to be altered from T-flask protocol. Note: Ensure that all cells have completely dissociated from the surface of the flask via observation under microscope. Failure to allow complete dissociation of cells from culture surface (such that all cells are freely floating) may result in reduced cell yields.
- If using trypsin, add desired volume of inactivating solution, such as serum-containing media.
- Collect cells either by aspirating or pouring, as in step 9.
法律信息
分析证书(COA)
输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。
难以找到您所需的产品或批次号码？
如何查找产品货号
在网站页面上，产品编号会附带包装尺寸/数量一起显示（例如：T1503-25G）。请确保 在“产品编号”字段中仅输入产品编号 (示例: T1503).
示例
其它示例：
705578-5MG-PW
PL860-CGA/SHF-1EA
MMYOMAG-74K-13
1000309185
输入内容 1.000309185)
遇到问题？欢迎随时联系我们技术服务 寻求帮助
如何查找COA批号
批号可以在产品标签上"批“ （Lot或Batch）字后面找到。
Aldrich 产品
如果您查询到的批号为 TO09019TO 等，请输入去除前两位字母的批号：09019TO。
如果您查询到的批号含有填充代码（例如05427ES-021），请输入去除填充代码-021的批号：05427ES。
如果您查询到的批号含有填充代码（例如 STBB0728K9），请输入去除填充代码K9的批号：STBB0728。
未找到您寻找的产品？
部分情况下，可能未在线提供COA。如果搜索不到COA，可在线索取。
相关内容
Monitor your cell cultures with ease with the Millicell® DCI Digital Cell Imager. From confluency to cell count and morphology, the Millicell® DCI automates cell culture measurements for quicker, more consistent culturing.
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门