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Merck
CN

PICM0RG50

Millicell器官培养型站立式培养小室, 亲水PTFE膜, 0.4 µm, 30 mm, 50/pk

pore size 0.4 μm, diam. 30 mm, transparent PTFE membrane, hydrophilic, H 5 mm, sterile

别名:

Millicell Cell Culture Insert, 30 mm, hydrophilic PTFE, 0.4 µm

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关于此项目

UNSPSC Code:
41104923
NACRES:
NB.22
eCl@ss:
32011202

主要文件

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产品名称

Millicell器官培养型站立式培养小室, 亲水PTFE膜, 0.4 µm, 30 mm, 50/pk, pore size 0.4 μm, diam. 30 mm, transparent PTFE membrane, hydrophilic, H 5 mm, sterile

material

transparent PTFE membrane
polystyrene housing (5 mm height)

sterility

ethylene oxide treated
sterile

feature

hydrophilic

manufacturer/tradename

Millicell®

packaging

pkg of Individually blister packaged

parameter

50 °C max. temp.

technique(s)

cell attachment: suitable
cell culture | mammalian: suitable
cell differentiation: suitable

H

5 mm

diam.

30 mm

size

6 wells

surface area

4.2 cm2

Quality Level

400

color

transparent membrane, when wetted

matrix

Biopore™

pore size

0.4 μm

binding type

low binding surface

detection method

fluorometric

shipped in

ambient

相关类别

Application

  • 细胞附着、3D 细胞培养、细胞生长、细胞分化、免疫细胞化学
  • Biopore 膜（亲水PTFE）是低蛋白结合、活细胞观察和免疫荧光应用的理想选择。

Features and Benefits

  • 三维外植体结构的高活力和优越性研究
  • 较低的高度令其适合装入标准培养皿
  • Biopore (PTFE) 膜提供高活力—长达40天—和良好的跨膜氧转运
  • 该膜是透明的，并针对器官型外植体的长期维护进行优化。

General description

凭借Millicell嵌套板，附着或悬浮细胞可以从其上下两侧接触到培养基。更加密切地模拟了体内所发生的细胞生长、结构和功能。另外，Millicell嵌套板可用于从两侧研究单层细胞。

Preparation Note

为确保膜从基底侧充分润湿，并减少气泡形成的几率，请按照以下步骤操作：
  1. 向接收孔中加入检测溶液或培养基：对于6孔板格式或等效尺寸，加入2 mL。
  2. 使用镊子以30°-45°的角度将插件放入接收孔中，以从基底侧逐渐润湿膜。
  3. 当插件边缘开始润湿时，轻轻放下插件，直至其完全嵌入孔中。

Legal Information

BIOPORE is a trademark of Merck KGaA, Darmstadt, Germany
Millicell is a registered trademark of Merck KGaA, Darmstadt, Germany

存储类别

10-13 - German Storage Class 10 to 13

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实验方案

Basic Indirect Co-culture on Both Sides of Millicell® Cell Culture Insert Membranes

This page covers the basic indirect co-culture procedure on both sides of Millicell cell culture insert membranes.

ECM Coating Protocols for Millicell®

This page covers the ECM coating protocols developed for four types of ECMs on Millicell®-CM inserts, Collagen Type 1, Fibronectin, Laminin, and Matrigel.

Microscopic Examination of Samples

This protocol covers 3 modes for the microscopic examination of cell samples.

Toluidine Blue Staining Protocol for Millicell® Inserts

Toluidine blue selectively stains nuclear material and acidic tissue components, aiding in histological staining for tissues rich in DNA/RNA.

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