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Merck
CN

PICM0RG50

Millipore

Millicell器官培养型站立式培养小室, 亲水PTFE膜, 0.4 µm, 30 mm, 50/pk

别名:

Millicell Cell Culture Insert, 30 mm, hydrophilic PTFE, 0.4 µm, Millicell-CM, cell culture inserts, organotypic inserts, plate inserts

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关于此项目

UNSPSC代码:
41104923
eCl@ss:
32011202
NACRES:
NB.22

主要文件

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质量水平

400

相关类别

一般描述

凭借Millicell嵌套板，附着或悬浮细胞可以从其上下两侧接触到培养基。更加密切地模拟了体内所发生的细胞生长、结构和功能。另外，Millicell嵌套板可用于从两侧研究单层细胞。

应用

  • 细胞附着、3D 细胞培养、细胞生长、细胞分化、免疫细胞化学
  • Biopore 膜（亲水PTFE）是低蛋白结合、活细胞观察和免疫荧光应用的理想选择。

特点和优势

  • 三维外植体结构的高活力和优越性研究
  • 较低的高度令其适合装入标准培养皿
  • Biopore (PTFE) 膜提供高活力—长达40天—和良好的跨膜氧转运
  • 该膜是透明的，并针对器官型外植体的长期维护进行优化。

制备说明

为确保膜从基底侧充分润湿，并减少气泡形成的几率，请按照以下步骤操作：
  1. 向接收孔中加入检测溶液或培养基：对于6孔板格式或等效尺寸，加入2 mL。
  2. 使用镊子以30°-45°的角度将插件放入接收孔中，以从基底侧逐渐润湿膜。
  3. 当插件边缘开始润湿时，轻轻放下插件，直至其完全嵌入孔中。

法律信息

BIOPORE is a trademark of Merck KGaA, Darmstadt, Germany
Millicell is a registered trademark of Merck KGaA, Darmstadt, Germany

储存分类代码

10-13 - German Storage Class 10 to 13

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

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Keisuke Ikegami et al.
Investigative ophthalmology & visual science, 61(3), 26-26 (2020-03-18)
Elevated IOP can cause the development of glaucoma. The circadian rhythm of IOP depends on the dynamics of the aqueous humor and is synchronized with the circadian rhythm pacemaker, that is, the suprachiasmatic nucleus. The suprachiasmatic nucleus resets peripheral clocks
Ryota Tamura et al.
Human gene therapy, 31(5-6), 352-366 (2020-02-23)
Glioblastoma is the most aggressive brain tumor characterized by diffuse infiltration into the normal brain parenchyma. Neural stem cells are known to possess the tumor-tropic migratory capacity and thus can be used as cellular vehicles for targeted delivery of therapeutic
Abigail K Myers et al.
Development (Cambridge, England), 147(2) (2020-01-10)
The precise migration of cortical interneurons is essential for the formation and function of cortical circuits, and disruptions to this key developmental process are implicated in the etiology of complex neurodevelopmental disorders, including schizophrenia, autism and epilepsy. We have recently
Gravity in mammalian organ development: differentiation of cultured lung and pancreas rudiments during spaceflight.
Spooner, B S, et al.
The Journal of Experimental Zoology, 269, 212-222 (1994)
Sara Elfarrash et al.
Acta neuropathologica communications, 7(1), 213-213 (2019-12-21)
Here we describe the use of an organotypic hippocampal slice model for studying α-synuclein aggregation and inter-neuronal spreading initiated by microinjection of pre-formed α-synuclein fibrils (PFFs). PFF injection at dentate gyrus (DG) templates the formation of endogenous α-synuclein aggregates in
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实验方案

Toluidine Blue Staining Protocol for Millicell® Inserts

Toluidine blue selectively stains nuclear material and acidic tissue components, aiding in histological staining for tissues rich in DNA/RNA.

Indirect Co-culture of Embryonic Stem Cells with Embryonic Fibroblasts

This page covers the indirect co-culture of embryonic stem cells with embryonic fibroblasts.

Microscopic Examination of Samples

This protocol covers 3 modes for the microscopic examination of cell samples.

Basic Indirect Co-culture on Both Sides of Millicell® Cell Culture Insert Membranes

This page covers the basic indirect co-culture procedure on both sides of Millicell cell culture insert membranes.

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