Merck
CN
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文件

PICM0RG50

Millipore

Millicell器官培养型站立式培养小室, 亲水PTFE膜, 0.4 µm, 30 mm, 50/pk

pore size 0.4 μm, diam. 30 mm, transparent PTFE membrane, hydrophilic, H 5 mm, size 6 wells, sterile

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别名:
可渗透培养小室, 组织培养小室, 组织培养板
eCl@ss:
32011202
NACRES:
NB.22

物料

polystyrene housing
transparent PTFE membrane

质量水平

400

无菌性

ethylene oxide treated
sterile

特点

hydrophilic

制造商/商品名称

Millicell®

包装

pkg of Individually blister packaged

参数

50 °C max. temp.

技术

cell attachment: suitable
cell culture | mammalian: suitable
cell differentiation: suitable

高度

5 mm

直径

30 mm

尺寸

6 wells

表面积

4.2 cm2

颜色

transparent, when wetted

基质

Biopore™

孔径

0.4 μm pore size

吸附类型

low binding surface

检测方法

fluorometric

运输

ambient

相关类别

一般描述

凭借Millicell®嵌套板，附着或悬浮细胞可以从其上下两侧接触到培养基。更加密切地模拟了体内所发生的细胞生长、结构和功能。此外，Millicell®嵌套板可实现对细胞单层的两侧都进行研究。

Millicell® Organtypic嵌套板：
- 用于实现高细胞活力以及针对三维外植体结构的出色研究
- 更低的高度使其能够放进标准的培养皿中
- Biopore（PTFE）膜可带来高活力—最长40天—以及极佳的跨膜氧气运输
- 膜是光学透明的，并经过优化可长期用于维持器官外植体

膜类型：
Biopore膜（亲水PTFE）
- 用于低蛋白结合、活细胞观察和免疫荧光应用

应用
细胞粘附、3D细胞培养、细胞生长、细胞分化、免疫细胞化学

干细胞类型：
小鼠胚胎干细胞
人胚胎干细胞
间充质干细胞
神经干细胞
造血干细胞
上皮细胞
胰腺干细胞
心脏干细胞
诱导性多能干细胞

应用

  • Cell Attachment, 3D Cell Culture, Cell Growth, Cell Differentiation , Immunocytochemistry
  • Biopore Membrane (hydrophilic PTFE) is ideal for low protein-binding, live cell viewing, and immunofluorescent applications

特点和优势

  • For high cell viability and superior study of three dimensional explant structure
  • Lower height allows them to fit inside a standard petri dish
  • The Biopore (PTFE) membrane provides high viability—for as long as 40 days—and excellent trans-membrane oxygen transport
  • The membrane is optically clear and optimized for long-term organotypic explant maintenance

法律信息

BIOPORE is a trademark of Merck KGaA, Darmstadt, Germany
Millicell is a registered trademark of Merck KGaA, Darmstadt, Germany

储存分类代码

10-13 - German Storage Class 10 to 13

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Keisuke Ikegami et al.
Investigative ophthalmology & visual science, 61(3), 26-26 (2020-03-18)
Elevated IOP can cause the development of glaucoma. The circadian rhythm of IOP depends on the dynamics of the aqueous humor and is synchronized with the circadian rhythm pacemaker, that is, the suprachiasmatic nucleus. The suprachiasmatic nucleus resets peripheral clocks
Ryota Tamura et al.
Human gene therapy, 31(5-6), 352-366 (2020-02-23)
Glioblastoma is the most aggressive brain tumor characterized by diffuse infiltration into the normal brain parenchyma. Neural stem cells are known to possess the tumor-tropic migratory capacity and thus can be used as cellular vehicles for targeted delivery of therapeutic
Abigail K Myers et al.
Development (Cambridge, England), 147(2) (2020-01-10)
The precise migration of cortical interneurons is essential for the formation and function of cortical circuits, and disruptions to this key developmental process are implicated in the etiology of complex neurodevelopmental disorders, including schizophrenia, autism and epilepsy. We have recently
P Hardman et al.
Development, growth & differentiation, 35(6), 683-690 (1993-01-01)
Embryonic mouse salivary glands, pancreata, and kidneys were isolated from embryos of appropriate gestational age by microdissection, and were cultured on Biopore membrane either non-coated or coated with type I collagen or Matrigel. As expected, use of Biopore membrane
R Le Bouffant et al.
Human reproduction (Oxford, England), 25(10), 2579-2590 (2010-07-31)
The initiation of meiosis is crucial to fertility. While extensive studies in rodents have enhanced our understanding of this process, studies in human fetal ovary are lacking. We used RT-PCR and immunohistochemistry to investigate expression of meiotic factors in human
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实验方案

Toluidine Blue Staining Protocol for Millicell® Culture Inserts

Toluidine blue stains the nuclear material in tissues with a high DNA and RNA content and is used for selectively staining acidic tissue components such as carboxylates, sulfates, and phosphate radicals.

Microscopic Examination of Samples

This protocol covers 3 modes for the microscopic examination of cell samples.

Indirect Co-culture of Embryonic Stem Cells with Embryonic Fibroblasts

This page covers the indirect co-culture of embryonic stem cells with embryonic fibroblasts.

Basic Indirect Co-culture on Both Sides of Millicell® Cell Culture Insert Membranes

This page covers the basic indirect co-culture procedure on both sides of Millicell cell culture insert membranes.

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