biological source
rabbit
Quality Segment
antibody form
purified antibody
antibody product type
primary antibodies
clone
polyclonal
form
liquid
does not contain
preservative
species reactivity
mouse, rat, chicken, human, Xenopus
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
isotype
IgG
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
phosphorylation (pTyr416)
Gene Information
human ... SRC(6714)
General description
Protein A and immunoaffinity purified rabbit polyclonal antibody. Recognizes the ~60 kDa Src protein phosphorylated at Tyr416.
Recognizes the ~60 kDa Src protein phosphorylated at Tyr416 in COLO 201 cells and PDGF-treated NIH/3T3 cells. May also recognize other Src family kinases phosphorylated at equivalent sites.
This PhosphoDetect Anti-Src Family (pTyr⁴¹⁶) Rabbit pAb is validated for use in Immunoblotting for the detection of Src Family (pTyr⁴¹⁶).
Immunogen
Human
a synthetic phosphopeptide corresponding to amino acids surrounding the Tyr⁴¹⁶ phosphorylation site of human Src
Application
Immunoblotting (1:1000)
Physical form
In 150 mM NaCl, 10 mM sodium HEPES, 100 µg/ml BSA, 50% glycerol, pH 7.5.
Preparation Note
Do not aliquot.
Analysis Note
Positive Control
COLO 201 cells, PDGF-treated NIH/3T3 cells, and breast carcinoma
COLO 201 cells, PDGF-treated NIH/3T3 cells, and breast carcinoma
Other Notes
May also recognize other Src family kinases phosphorylated at equivalent sites. The immunogen sequence is 100% conserved in chicken, rat, and Xenopus, but cross-reactivity has not been tested. For immunoblotting the membrane should be incubated with antibody diluted in 5% BSA, 1X TBS, 0.1% Tween®-20 detergent at 4°C overnight with gentle shaking. Antibody should be titrated for optimal results in individual systems.
Thomas, S.M. and Brugge, J.S. 1997. Annu. Rev. Cell Dev. Biol.13, 513.
Chow, L.M. et al. 1993. Nature365, 156.
Molina, T.J., et al. 1992. Nature357, 161.
Straus, D.B. and Weiss, A. 1992. Cell70, 585.
Yamanashi, Y., et al. 1991. Science251, 192.
Yamanashi, Y., et al. 1989. Proc. Natl. Acad. Sci. USA86, 6538.
Hunter, T. 1987. Cell49, 1.
Chow, L.M. et al. 1993. Nature365, 156.
Molina, T.J., et al. 1992. Nature357, 161.
Straus, D.B. and Weiss, A. 1992. Cell70, 585.
Yamanashi, Y., et al. 1991. Science251, 192.
Yamanashi, Y., et al. 1989. Proc. Natl. Acad. Sci. USA86, 6538.
Hunter, T. 1987. Cell49, 1.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
TWEEN is a registered trademark of Croda International PLC
Disclaimer
Toxicity: Standard Handling (A)
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存储类别
10 - Combustible liquids
wgk
WGK 2