产品名称
PhosphoDetect Anti-RhoA (pSer¹⁸⁸) Rabbit pAb, liquid, Calbiochem®
biological source
rabbit
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity
human
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
dilution
(Immunoblotting (1:1000)
ELISA (1:2000))
isotype
IgG
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
phosphorylation (pSer188)
Quality Level
Gene Information
human ... RHOA(387)
Analysis Note
Positive Control
RhoA GST fusion protein
RhoA GST fusion protein
Application
Immunoblotting (1:1000)
ELISA (1:2000)
Disclaimer
Toxicity: Standard Handling (A)
General description
Immunoaffinity purified rabbit polyclonal antibody. Recognizes the ~22 kDa RhoA protein when expressed as a GST fusion protein (total ~49 kDa) and phosphorylated in vitro by PKA.
Recognizes the ~22 kDa RhoA protein, expressed as a GST fusion protein (total ~49 kDa) and phosphorylated in vitro by PKA.
This PhosphoDetect Anti-RhoA (pSer¹⁸⁸) Rabbit pAb is validated for use in Immunoblotting, ELISA for the detection of RhoA (pSer¹⁸⁸).
Immunogen
Human
a synthetic peptide corresponding to amino acids surrounding the Ser¹⁸⁸ phosphorylation site of human RhoA, coupled to KLH
Other Notes
Does not recognize unphosphorylated RhoA. The immunogen sequence is conserved in rat and mouse RhoA, but cross-reactivity has not been tested. The immunogen sequence is not found in the RhoB and RhoC sequences. For immunoblotting, the membrane was incubated with antibody diluted in PBS/5% nonfat milk/0.04% Tween® 20 detergent for 1 h at room temperature. Antibody should be titrated for optimal results in individual systems.
Ellerbroek, S.M., et al. 2003. J. Biol. Chem.278, 19023.
Sawada, N., et al. 2001. Biochem. Biophys. Res. Commun. 280, 798.
Dong, J.M., et al. 1998. J. Biol. Chem.273, 22554.
Lang, P., et al. 1996. EMBO J.15, 510.
Sawada, N., et al. 2001. Biochem. Biophys. Res. Commun. 280, 798.
Dong, J.M., et al. 1998. J. Biol. Chem.273, 22554.
Lang, P., et al. 1996. EMBO J.15, 510.
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In PBS, 1 mg/ml BSA, 50% glycerol.
Preparation Note
Do not aliquot.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
TWEEN is a registered trademark of Croda International PLC
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存储类别
10 - Combustible liquids
wgk
WGK 1
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