PSHT004S5
Millicell® 96孔板
pore size 0.4 μm, polycarbonate membrane, sterile, Tissue Culture (TC)-treated surface, automation compatible, pack of 5 × with 96-well receiver plate
别名:
96孔Millicell板, Millicell板
物料
polycarbonate membrane
polystyrene plate
质量水平
描述
Components: 96 well cell culture plate (5), 96 well receiver plate (10), Lid (5)
无菌性
γ-irradiated
sterile
特点
automation compatible
制造商/商品名称
Millicell®
包装
pack of 5 × with 96-well receiver plate
技术
cell attachment: suitable
cell culture | mammalian: suitable
cell differentiation: suitable
drug transporter assay: suitable
permeability assay: suitable
toxicology assay: suitable
长度 × 宽度 × 高度
127.76 mm × 85.47 mm × 20.32 mm, filter plate and receiver plate
过滤面积
0.11 cm2
板尺寸
96 wells
工作体积
0.075 mL
基质
Isopore™
孔径
0.4 μm
吸附类型
Tissue Culture (TC)-treated surface
运输
ambient
一般描述
Millicell装置有0.4 µm PCF和多种PET薄膜孔径。培养板给用户提供了最大方便性,有许多特点,例如更加方便移液的上侧辅助孔以及下侧接触孔。为了降低污染细胞单层的风险,薄膜板有支脚,使板子在从饲养托盘拆下时高于工作表面。泪滴形状的容纳孔消除了在组装板子时产生的气泡。
Millicell培养板经优化用于生长细胞和维持细胞单层的高度完整性。Millicell培养板薄膜上生长的细胞的生长情况比在塑料上好,因为细胞从上侧和下侧均获得营养。更加密切地模拟了体内细胞生长和功能。Millicell培养板设计用于分析及细胞生长,亦可用作手动或自动细胞接种、饲养和清洗系统。
使用这一完善的系统,您可以在一个以薄膜做底的培养板上生长、饲养和分析细胞。培养板可以与单槽或多孔饲养托盘配用。在进行转运分析时,只需将培养板转换成分析用的多孔转运托盘。
这种流线型设计增强了下列功能:
-接种和饲养系统
-自动液体处理系统和跨距移液
-下侧和上侧均可接触到细胞
-经上皮电阻(TEER)测量系统
特点和优势
- Enhanced Cell Growth: Cells cultured on Millicell plates exhibit better growth compared to traditional plastic, as they receive nutrients from both the apical and basolateral sides, mimicking in vivo conditions.
- User-Friendly Design: Incorporates apical assist for easy pipetting and basolateral access. Tear drop-shaped receiver wells prevent air bubbles during assembly.
- Streamlined Workflow: The complete system allows for simultaneous growth, feeding, and analysis within one membrane-bottom plate, simplifying workflows.
- Automation Compatible: Designed to work seamlessly with seed-and-feed systems, automated liquid handling, and TEER measurement systems, enhancing laboratory efficiency.
法律信息
商品
Application note on Drug transport assays in a 96-well system using Millicell-96 System from Millipore.
实验方案
Toluidine blue selectively stains nuclear material and acidic tissue components, aiding in histological staining for tissues rich in DNA/RNA.
This protocol covers 3 modes for the microscopic examination of cell samples.
This page covers the basic indirect co-culture procedure on both sides of Millicell cell culture insert membranes.
This page covers the ECM coating protocols developed for four types of ECMs on Millicell®-CM inserts, Collagen Type 1, Fibronectin, Laminin, and Matrigel.
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