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Merck
CN

PSHT004S5

Millipore

Millicell® 96孔板

pore size 0.4 μm, polycarbonate membrane, sterile, Tissue Culture (TC)-treated surface, automation compatible, pack of 5 × with 96-well receiver plate

别名:

96孔Millicell板, Millicell板

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关于此项目

UNSPSC代码:
41104923
eCl@ss:
32039006
NACRES:
NB.13
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物料

polycarbonate membrane
polystyrene plate

质量水平

描述

Components: 96 well cell culture plate (5), 96 well receiver plate (10), Lid (5)

无菌性

γ-irradiated
sterile

特点

automation compatible

制造商/商品名称

Millicell®

包装

pack of 5 × with 96-well receiver plate

技术

cell attachment: suitable
cell culture | mammalian: suitable
cell differentiation: suitable
drug transporter assay: suitable
permeability assay: suitable
toxicology assay: suitable

长度 × 宽度 × 高度

127.76 mm × 85.47 mm × 20.32 mm, filter plate and receiver plate

过滤面积

0.11 cm2

板尺寸

96 wells

工作体积

0.075 mL

基质

Isopore

孔径

0.4 μm

吸附类型

Tissue Culture (TC)-treated surface

运输

ambient

一般描述

Millicell细胞培养嵌套板是一种专利装置,用于支持内皮和上皮细胞系(包括CaCo-2)的细胞生长和分化。

Millicell装置有0.4 µm PCF和多种PET薄膜孔径。培养板给用户提供了最大方便性,有许多特点,例如更加方便移液的上侧辅助孔以及下侧接触孔。为了降低污染细胞单层的风险,薄膜板有支脚,使板子在从饲养托盘拆下时高于工作表面。泪滴形状的容纳孔消除了在组装板子时产生的气泡。

Millicell培养板经优化用于生长细胞和维持细胞单层的高度完整性。Millicell培养板薄膜上生长的细胞的生长情况比在塑料上好,因为细胞从上侧和下侧均获得营养。更加密切地模拟了体内细胞生长和功能。Millicell培养板设计用于分析及细胞生长,亦可用作手动或自动细胞接种、饲养和清洗系统。

使用这一完善的系统,您可以在一个以薄膜做底的培养板上生长、饲养和分析细胞。培养板可以与单槽或多孔饲养托盘配用。在进行转运分析时,只需将培养板转换成分析用的多孔转运托盘。

这种流线型设计增强了下列功能:

-接种和饲养系统

-自动液体处理系统和跨距移液

-下侧和上侧均可接触到细胞

-经上皮电阻(TEER)测量系统

特点和优势

  • Enhanced Cell Growth: Cells cultured on Millicell plates exhibit better growth compared to traditional plastic, as they receive nutrients from both the apical and basolateral sides, mimicking in vivo conditions.
  • User-Friendly Design: Incorporates apical assist for easy pipetting and basolateral access. Tear drop-shaped receiver wells prevent air bubbles during assembly.
  • Streamlined Workflow: The complete system allows for simultaneous growth, feeding, and analysis within one membrane-bottom plate, simplifying workflows.
  • Automation Compatible: Designed to work seamlessly with seed-and-feed systems, automated liquid handling, and TEER measurement systems, enhancing laboratory efficiency.

法律信息

ISOPORE is a trademark of Merck KGaA, Darmstadt, Germany
Millicell is a registered trademark of Merck KGaA, Darmstadt, Germany

分析证书(COA)

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商品

Application note on Drug transport assays in a 96-well system using Millicell-96 System from Millipore.

实验方案

Toluidine blue selectively stains nuclear material and acidic tissue components, aiding in histological staining for tissues rich in DNA/RNA.

This protocol covers 3 modes for the microscopic examination of cell samples.

This page covers the basic indirect co-culture procedure on both sides of Millicell cell culture insert membranes.

This page covers the ECM coating protocols developed for four types of ECMs on Millicell®-CM inserts, Collagen Type 1, Fibronectin, Laminin, and Matrigel.

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