用途
sufficient for 500 tests
质量水平
包装
pkg of 1 96-well plate(s)
制造商/商品名称
Calbiochem®
储存条件
OK to freeze
avoid repeated freeze/thaw cycles
protect from light
输入
sample type cultured cells (1,000-50,000 cells)
检测方法
colorimetric
运输
wet ice
储存温度
−20°C
一般描述
Assay measures the increased activity of cellular mitochondrial dehydrogenases that can cleave the tetrazolium dye WST-1 to formazan. The formazan formation is then quantified by measuring the change in absorbance at 450 nm in a microplate reader. The activity of mitochondrial dehydrogenases is proportional to cell number. No washing, harvesting, or solubilization steps are required.
生化/生理作用
The Calbiochem Rapid Cell Proliferation Kit is designed to determine the number of viable cells in proliferation, or cytotoxicity studies, based on the cleavage of the tetrazolium salt WST-1 by mitochondrial dehydrogenases in viable cells. The assay can be used to measure cell proliferation in response to growth factors or cytokines, or to assess cytotoxic compounds.
特点和优势
Assay Time: 0.5 - 4 h
Sensitivity: 1000 cells (adherent); 2500 cells (nonadherent)
制备说明
• WST-1 Labeling Mixture: Thaw the EMS solution at room temperature or in a 37°C water bath. Add the entire contents of this solution to the bottle containing the WST-1 reagent. The mixed solution can be stored at 4°C for several weeks. For long-term storage (several months) aliquot and store at -20°C. Protect from light.
其他说明
Ishiyama, M., et al. 1995. In Vitro Toxicology8, 187.
Liu, S.O., et al 1995. Nat. Med.1, 267.
Ishiyama, M., et al. 1993. Chem. Pharm. Bull.41, 1118.
Liu, S.O., et al 1995. Nat. Med.1, 267.
Ishiyama, M., et al. 1993. Chem. Pharm. Bull.41, 1118.
WST-1, 1-Methoxy PMS, and a user protocol.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Toxicity: Multiple Toxicity Values, refer to MSDS (O)
储存分类代码
10 - Combustible liquids
法规信息
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商品
Cell based assays for cell proliferation (BrdU, MTT, WST1), cell viability and cytotoxicity experiments for applications in cancer, neuroscience and stem cell research.
基于细胞的细胞增殖（BrdU、MTT、WST1）、细胞活力和细胞毒性实验，可应用于癌症、神经科学和干细胞研究。
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