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Merck
CN

S2EM004M99

Millipore

Multiscreen® 96孔板,疏水性PVDF膜

pore size 0.45 μm, sterile

别名:

PVDF 96孔板, 疏水性96孔板

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UNSPSC代码:
41104923
eCl@ss:
32039006
NACRES:
NB.22
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物料

PVDF membrane (hydrophobic)
acrylic

质量水平

描述

Sterile, white 96-well filter plate with 0.45 um pore size Hydrophobic PVDF membrane for ELISPOT Assays. Comes in a pack of 10.

无菌性

sterile

制造商/商品名称

MultiScreen®

参数

50-250 μL sample volume (per well)

技术

ELISPOT: suitable

过滤面积

0.28 cm2

板尺寸

96 wells

基质

Immobilon®-P

孔径

0.45 μm pore size

运输

ambient

一般描述

滤器编码:IP

应用

MultiScreen®-IP 白色无菌过滤板, 0.45 μm已被用于酶联免疫斑点(ELISPOT)试验。

外形

色码:白色

其他说明

自动化兼容性:否

法律信息

Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany
MULTISCREEN is a registered trademark of Merck KGaA, Darmstadt, Germany

分析证书(COA)

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Sarah Côté-Bigras et al.
Clinical endocrinology, 80(2), 296-300 (2013-06-06)
While thyrotrophin receptor (TSHR) is recognized as the main autoantigen in Graves' disease (GD), the actual antigen specificity of T cells that infiltrate the thyroid and the orbit is unknown. Identifying T cell responses to TSHR peptides has been difficult
Daniela Frasca et al.
Vaccine, 34(25), 2834-2840 (2016-04-25)
The success of a vaccine in inducing a protective antibody response depends on the longevity of both long-lived plasma cells (PC) and memory B cells. We have previously shown that the in vivo antibody response to a new influenza vaccine
Sung-Ho Chang et al.
Cells, 9(5) (2020-05-24)
We investigated the immunogenicity of allogeneic human adipose-derived mesenchymal stem cells (ADSCs) through the production of alloreactive-CD8 T and -memory CD8 T cells, based on their human leukocyte antigen (HLA) expression. In surface antigen analysis, ADSCs do not express co-stimulatory
Kee Thai Yeo et al.
Journal of immunology (Baltimore, Md. : 1950), 202(5), 1465-1478 (2019-01-25)
Infections during pregnancy can expose the fetus to microbial Ags, leading to inflammation that affects B cell development. Prenatal fetal immune priming may have an important role in infant acquisition of pathogen-specific immunity. We examined plasma proinflammatory biomarkers, the proportions
Samantha K Greaney et al.
Cancer immunology research, 8(2), 246-254 (2019-12-20)
Whereas systemic IL12 is associated with potentially life-threatening toxicity, intratumoral delivery of IL12 through tavokinogene telseplasmid electroporation (tavo) is safe and can induce tumor regression at distant sites. The mechanism by which these responses are mediated is unknown but is

商品

ELISpot assay provides qualitative and quantitative information on immune responses, visualizing multiple secretory products from single responding cells.

实验方案

The ELISpot (Enzyme Linked Immuno-Spot) assay provides an effective method of measuring the antibody or cytokine production of immune cells on the single cell level.

Guidelines for various bioassays on the MultiScreen system, which makes it possible to carry out an assay from sample incubation to scintillation counting in a 96-well plate.

相关内容

The ELISpot (Enzyme Linked Immuno-Spot) assay provides an effective method of measuring the antibody or cytokine production of immune cells on the single cell level. The popularity of this assay has seen resurgence in recent years as researchers attempt to gain a better understanding of immune responses in a variety of applications. The following protocol is an example of a typical ELISpot assay for quantifying the number cells producing interferon-gamma (IFN-γ) in response to antigen or non-specific activation using phytohemagglutinin (PHA). It may be optimized as necessary for other applications.

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