manufacturer/tradename
Chemicon®
Quality Segment
technique(s)
activity assay: suitable (apoptosis)
detection method
fluorometric
shipped in
dry ice
General description
Propidium Iodide: {3,8-diamino-5-[3-(diethylmethylammonio)propyl]-6-phenylphenanthridinium diodide}. Antifade Solution: p-Phenylenediamine and glycerol in PBS solution. 2.5μg/mL Propidium Iodide (PI) suspended in Antifade.
Application
Propidium Iodide is a nuclear fluorescent stain used for counterstaining paraffin-embedded, formalin-fixed tissue, to discriminate between live vs. dead cells in flow cytometry, and staining metaphase chromosomes or interphase nuclei that have been hybridized with probes detected with Fluorescein, or equivalent fluors. Antifade prevents the permanent loss of fluorescence or fluorescent signal quenching due to photobleaching and prolonged exposure to high intensity light sources.
Materials Provided
Each vial contains 1 ml of Propidium Iodide/Antifade Solution.
Warnings and Precautions
Propidium Iodide is a potential carcinogen. Antifade is a potential toxin and irritant. Use extreme caution. Avoid contact with eyes, skin and mucous membranes. Wear gloves and a lab coat when handling. Follow your institution′s guidelines for hazardous waste disposal. Keep tightly capped and limit exposure to light.
Related Products
Antifade Solution S7114
DAPI/Antifade Solution S7113
Propidium Iodide Solution S7109
Materials Provided
Each vial contains 1 ml of Propidium Iodide/Antifade Solution.
Warnings and Precautions
Propidium Iodide is a potential carcinogen. Antifade is a potential toxin and irritant. Use extreme caution. Avoid contact with eyes, skin and mucous membranes. Wear gloves and a lab coat when handling. Follow your institution′s guidelines for hazardous waste disposal. Keep tightly capped and limit exposure to light.
Related Products
Antifade Solution S7114
DAPI/Antifade Solution S7113
Propidium Iodide Solution S7109
Preparation Note
Recommended Storage
-15ºC to -25ºC
Protect from light
-15ºC to -25ºC
Protect from light
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.